N-乙酰基神经鞘氨醇

生物活性靶点体外研究体内研究 N-乙酰基神经鞘氨醇试剂级价格

N-乙酰基神经鞘氨醇

CAS号:3102-57-6
英文名:C2 CERAMIDE
中文名:N-乙酰基神经鞘氨醇
CBNumber:CB8342539
分子式:C20H39NO3
分子量:341.53
MOL File:3102-57-6.mol

N-乙酰基神经鞘氨醇化学性质

熔点 :93-96 °C
沸点 :477.13°C (rough estimate)
密度 :1.0283 (rough estimate)
折射率 :1.5614 (estimate)
储存条件 :−20°C
溶解度 :DMSO: soluble
酸度系数(pKa) :13.50±0.20(Predicted)
形态 :powder
颜色 :white
稳定性 :Moisture Sensitive

安全信息

安全说明 :22-24/25
WGK Germany :3
海关编码 :29241990

N-乙酰基神经鞘氨醇性质、用途与生产工艺

生物活性 C2 Ceramide (Ceramide 2) 是一种角质层的主要脂质，也是一种蛋白质磷酸酶 1 (PP1) 的激活剂。C2 Ceramide 还可激活 PP2A 和神经酰胺激活的蛋白磷酸酶 (CAPP)。C2 Ceramide 可诱导细胞分化和凋亡 (apoptosis)，抑制线粒体呼吸链复合物 III (mitochondrial respiratory chain complex III)。C2 Ceramide 是一种皮肤调理剂，可保护表皮屏障免受水分流失。
靶点

Protein phosphatase 1
PP2A
Ceramide-activated protein phosphatase (CAPP)
Apoptosis
Mitochondrial respiratory chain complex III

体外研究

C2 Ceramide (5 nM-200 µM; 24 hours; primary mouse osteoblasts) treatment (≤500 nM) promots osteoblast viability, whilst concentrations ≥2 µM significantly reduces osteoblast viability in a dose- and time-dependent manner.
C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50 µM and 100 µM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts.
C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix.

Cell Viability Assay

Cell Line:	Primary mouse osteoblasts
Concentration:	5 nM-200 µM
Incubation Time:	24 hours
Result:	Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n M. Increasing concentrations of 20-200µM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival.

体内研究

The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation.