IBERIOTOXIN毒素肽

生物活性靶点体外研究体内研究 IBERIOTOXIN毒素肽试剂级价格

IBERIOTOXIN毒素肽

CAS号:129203-60-7
英文名:IBERIOTOXIN
中文名:IBERIOTOXIN毒素肽
CBNumber:CB8130448
分子式:C179H274N50O55S7
分子量:4230.84786
MOL File:129203-60-7.mol

IBERIOTOXIN毒素肽化学性质

储存条件 :-20°C
溶解度 :H₂O: 1 mg/mL, clear, colorless
形态 :Salt-free lyophilized solid
水溶解性 :Soluble to 0.70 mg/ml in water

安全信息

危险品标志 :T+
危险类别码 :26/27/28
安全说明 :28-36/37-45
危险品运输编号 :UN 3462 6.1/PG 2
WGK Germany :3
RTECS号 :NH6107500
F :3-10

IBERIOTOXIN毒素肽性质、用途与生产工艺

生物活性 Iberiotoxin 是一种从 Buthus tamulus 毒液中分离出来的毒素。Iberiotoxin 是一种选择性的高电导率 Ca2+ 激活的 K+ 通道 (Ca2+-activated K+ channel) 抑制剂，Kd 约为 1 nM。Iberiotoxin 不阻断其他类型的电压依赖性离子通道。
靶点

Kd: 1 nM (Ca ²⁺ -activated K ⁺ channel)
体外研究

Iberiotoxin reversibly blocks Ca2+-activated K+ channel in excised membrane patches from bovine aortic smooth muscle. Iberiotoxin acts exclusively at the outer face of the channel and functions with IC ₅₀ values of about 250 pM. Iberiotoxin is a partial inhibitor of ¹²⁵ I-charybdotoxin binding in bovine aortic sarcolemmal membrane vesicles (Ki of 2 ₅₀ pM). Iberiotoxin functions as a noncompetitive inhibitor of charybdotoxin binding.
Iberiotoxin treatment affects rat mesenchymal stem cells (rMSCs) migration in the absence of platelet lysate (PL) by inducing a decrease in cell migration suggesting that BK channels regulate rMSCs migration in basal conditions. 10 nM of Iberiotoxin abolishes the PL-induced migration effect on MSCs.
体内研究

Male Wistar rats (6-7 weeks old) with chronic heart failure (CHF), Iberiotoxin (0.125 nmol/nl, 1.25 nmol/nl and 12.5 nmol/nl) is infused into paraventricular hypothalamic nucleus (PVN) by osmotic minipumps. After perfusion of Iberiotoxin by microinjection of rAAV2-KCNMB4 shRNA virus, right ventricular weight/body weight and lung weight/body weight ratio as well as left ventricular end-diastolic diameter are increased and left ventricular ejection fraction is decreased.