生物活性 靶点 体外研究 NSC 95397 试剂级价格
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NSC 95397

NSC 95397,93718-83-3,结构式
NSC 95397
  • CAS号:93718-83-3
  • 英文名:NSC 95397
  • 中文名:NSC 95397
  • CBNumber:CB7402539
  • 分子式:C14H14O4S2
  • 分子量:310.39
  • MOL File:93718-83-3.mol
NSC 95397化学性质
  • 储存条件 :-20°C
  • 溶解度 :DMSO: 16 mg/mL
  • 形态 :Orange solid
  • 颜色 :orange, solid
  • 稳定性 :Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
安全信息
  • 危险品标志 :Xi
  • WGK Germany :3
  • 危险等级 :IRRITANT

NSC 95397性质、用途与生产工艺

  • 生物活性 NSC 95397 是一种有效的、选择性 Cdc25 双特异性磷酸酶抑制剂,对于 Cdc25A、Cdc25B 和 Cdc25C 的Ki值分别为32 nM、96 nM 和 40 nM。NSC 95397 对 hCdc25A、hCdc25C 和 hCdc25B 的IC50值分别为22.3 nM、56.9 nM和125 nM。NSC 95397 抑制 mitogen-activated protein kinase phosphatase-1 (MKP-1) 和增殖,并通过 MKP-1 和 ERK1/2 途径诱导结肠癌细胞凋亡。
  • 靶点
    TargetValue
    MKP-1
    ()
    Cdc25A
    (Cell-free assay)
    22.3 nM
    Cdc25A
    (Cell-free assay)
    32 nM(Ki)
    Cdc25C
    (Cell-free assay)
    40 nM(Ki)
    Cdc25B
    (Cell-free assay)
    56.9 nM
  • 体外研究

    NSC 95397 (0, 10, and 20 µM; 24 hour) decreases the cell viability of three colon cancer cell lines SW480, SW620, and DLD-1 in a concentration-dependent manner.
    NSC 95397(10 μM; 24 hour) upregulates p21 while downregulates CDK4 and CDK6 were d in all three colon cancer cell lines SW480, SW620, and DLD-1 cells.
    NSC 95397 (10 μM; 24 hour) reduces the phosphorylation of retinoblastoma protein (Rb) on Ser795 and Ser807/811.
    NSC 95397 (20 μM; 24 hours) significantly increases cleaved caspase-9, -3, -7 and PARP levels.
    NSC 95397 (10 μM; 6 hours) enhances the phosphorylation of its downstream ERK1/2 at Thr202/Tyr 204.

    Cell Viability Assay

    Cell Line: Human colon cancer cell lines, SW480, SW620, and DLD-1
    Concentration: 0, 10, and 20 µM
    Incubation Time: 24 hours
    Result: The IC 50 values of NSC 95397 for the cell growth of SW480, SW620, and DLD-1 cells were 9.9, 14.1 and 18.6 μM, respectively.

    Western Blot Analysis

    Cell Line: SW480, SW620, and DLD-1 cells
    Concentration: 10 μM
    Incubation Time: 24 hours
    Result: p21 was upregulated while CDK4 and CDK6 were downregulated.
    Rduced the phosphorylation of Rb on Ser795 and Ser807/811
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NSC 95397 试剂级价格
  • 更新日期:2024/11/08
  • 产品编号:HY-108543
  • 产品名称:NSC 95397 NSC 95397
  • CAS编号:93718-83-3
  • 包装:5mg
  • 价格:1200元
  • 更新日期:2024/11/08
  • 产品编号:HY-108543
  • 产品名称:NSC 95397 NSC 95397
  • CAS编号:93718-83-3
  • 包装:10mM * 1mLin DMSO
  • 价格:1320元
NSC 95397生产厂家
  • 公司名称:TOCRIS-US
  • 联系电话:--
  • 电子邮件:customerservice@tocris.co.uk
  • 国家:英国
  • 产品数:5726
  • 优势度:77
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