ChemicalBook CAS数据库列表 NSC 95397
NSC 95397
- CAS号:93718-83-3
- 英文名:NSC 95397
- 中文名:NSC 95397
- CBNumber:CB7402539
- 分子式:C14H14O4S2
- 分子量:310.39
- MOL File:93718-83-3.mol
NSC 95397化学性质
- 储存条件 :-20°C
- 溶解度 :DMSO: 16 mg/mL
- 形态 :Orange solid
- 颜色 :orange, solid
- 稳定性 :Stable for 1 year from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
安全信息
- 危险品标志 :Xi
- WGK Germany :3
- 危险等级 :IRRITANT
NSC 95397性质、用途与生产工艺
- 生物活性 NSC 95397 是一种有效的、选择性 Cdc25 双特异性磷酸酶抑制剂，对于 Cdc25A、Cdc25B 和 Cdc25C 的Ki值分别为32 nM、96 nM 和 40 nM。NSC 95397 对 hCdc25A、hCdc25C 和 hCdc25B 的IC50值分别为22.3 nM、56.9 nM和125 nM。NSC 95397 抑制 mitogen-activated protein kinase phosphatase-1 (MKP-1) 和增殖，并通过 MKP-1 和 ERK1/2 途径诱导结肠癌细胞凋亡。
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靶点
Target Value MKP-1
()Cdc25A
(Cell-free assay)22.3 nM Cdc25A
(Cell-free assay)32 nM(Ki) Cdc25C
(Cell-free assay)40 nM(Ki) Cdc25B
(Cell-free assay)56.9 nM -
体外研究
NSC 95397 (0, 10, and 20 µM; 24 hour) decreases the cell viability of three colon cancer cell lines SW480, SW620, and DLD-1 in a concentration-dependent manner.
NSC 95397(10 μM; 24 hour) upregulates p21 while downregulates CDK4 and CDK6 were d in all three colon cancer cell lines SW480, SW620, and DLD-1 cells.
NSC 95397 (10 μM; 24 hour) reduces the phosphorylation of retinoblastoma protein (Rb) on Ser795 and Ser807/811.
NSC 95397 (20 μM; 24 hours) significantly increases cleaved caspase-9, -3, -7 and PARP levels.
NSC 95397 (10 μM; 6 hours) enhances the phosphorylation of its downstream ERK1/2 at Thr202/Tyr 204.Cell Viability Assay
Cell Line: Human colon cancer cell lines, SW480, SW620, and DLD-1 Concentration: 0, 10, and 20 µM Incubation Time: 24 hours Result: The IC 50 values of NSC 95397 for the cell growth of SW480, SW620, and DLD-1 cells were 9.9, 14.1 and 18.6 μM, respectively. Western Blot Analysis
Cell Line: SW480, SW620, and DLD-1 cells Concentration: 10 μM Incubation Time: 24 hours Result: p21 was upregulated while CDK4 and CDK6 were downregulated.
Rduced the phosphorylation of Rb on Ser795 and Ser807/811
NSC 95397上下游产品信息
上游原料
下游产品
NSC 95397 试剂级价格
- 更新日期：2024/08/19
- 产品编号：HY-108543
- 产品名称：NSC 95397 NSC 95397
- CAS编号：93718-83-3
- 包装：5mg
- 价格：1200元
- 更新日期：2024/08/19
- 产品编号：HY-108543
- 产品名称：NSC 95397 NSC 95397
- CAS编号：93718-83-3
- 包装：10mM * 1mLin DMSO
- 价格：1320元
NSC 95397生产厂家
- 公司名称：TOCRIS-US
- 联系电话：--
- 电子邮件：customerservice@tocris.co.uk
- 国家：英国
- 产品数：5726
- 优势度：77
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