金黄紫堇碱

用途生物活性体外研究金黄紫堇碱试剂级价格

金黄紫堇碱

CAS号:6451-73-6
英文名:SCOULERIN
中文名:金黄紫堇碱
CBNumber:CB7382903
分子式:C19H21NO4
分子量:327.37
MOL File:6451-73-6.mol

金黄紫堇碱化学性质

熔点 :192°C
沸点 :503.3±50.0 °C(Predicted)
密度 :1.37±0.1 g/cm3(Predicted)
储存条件 :Store at -20°C
溶解度 :Soluble in DMSO
形态 :A crystalline solid
酸度系数(pKa) :9.88±0.20(Predicted)

金黄紫堇碱性质、用途与生产工艺

用途金黄紫堇碱是完整的碳环。它不仅是提供原小檗碱类生物碱合成的基本骨架，也是许多生物碱合成的前体物质，例如小檗碱以及苯并菲啶类生物碱。
生物活性 Scoulerine ((-)-Scoulerine) 是一种有效的抗有丝分裂 (antimitotic) 化合物。Scoulerine 也是 BACE1 (淀粉样前体蛋白裂解酶 1) 的抑制剂。Scoulerine 抑制细胞增殖，阻止细胞周期，并诱导癌细胞凋亡。

体外研究

Scoulerine ((-)-Scoulerine) inhibits mini-panel of human leukemic cells, MOLT-4 (WT), Jurkat (TP53 mutated), Raji (TP53 mutated), HL-60 (TP53 null), U-937 (TP53 mutated), and HEL 92.1.7 (wild-type), with IC ₅₀ s ranging from 2.7 µM to 6.5 µM.
Scoulerine (2.5-20 µM; 24 hours) decreases proliferation of Jurkat and MOLT-4 cells.
Scoulerine (2.5-20 µM; 24 hours) induces MOLT-4 and Jurkat cells apoptosis.
Scoulerine induces G2 or M cell cycle arrest.
Scoulerine (2.5-5 µM; 24 hours) shows an upregulation of p53 protein in p53 wild-type MOLT-4 cells.
Scoulerine (2.5-5 µM; 24-48 hours) activates caspase-3/7, -8 and -9 in a dose-dependent manner.
Scoulerine (5-10 µM; 24 hours) disrupts microtubule structure of A549 lung carcinoma cells

Cell Proliferation Assay

Cell Line:	Jurkat and MOLT-4 cells
Concentration:	2.5, 5, 10, 15 and 20 µM
Incubation Time:	24 hours
Result:	Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner.