二苯基氯化碘盐

生物活性靶点体外研究体内研究二苯基氯化碘盐试剂级价格

二苯基氯化碘盐

CAS号:4673-26-1
英文名:DIPHENYLENEIODONIUM CHLORIDE
中文名:二苯基氯化碘盐
CBNumber:CB7355273
分子式:C12H8ClI
分子量:314.55
MOL File:4673-26-1.mol

二苯基氯化碘盐化学性质

熔点 :312-322 °C
储存条件 :room temp
溶解度 :Soluble in DMSO (up to 5 mg/ml with warming)
形态 :Powder
颜色 :Off-white
稳定性 :Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 2 months.

安全信息

危险品标志 :Xi
危险类别码 :36
安全说明 :26
WGK Germany :3
海关编码 :29310099

二苯基氯化碘盐性质、用途与生产工艺

生物活性 Diphenyleneiodonium chloride (DPI)是 NADPH oxidase 的抑制剂，也是有效的、不可逆的 iNOS/eNOS 抑制剂。Diphenyleneiodonium chloride (DPI) 还起到 TRPA1 激活剂的作用，并能选择性地抑制细胞内的活性氧(ROS)。
靶点
Target Value
NADPH oxidase
()
体外研究
DPI抑制NADPH oxidase、一氧化氮合成酶、黄嘌呤氧化酶和NADPH细胞色素P450氧化还原酶的活性。飞摩尔级别的DPI即可通过抑制ERK调节的PHOX活性、抑制小胶质细胞的激活，发挥其抗炎活性和神经保护作用。DPI常被用于抑制由多种黄素酶介导的ROS生成。NADPH, NADP+, and 2'5'-ADP可阻止DPI的抑制活性。对ARPE-19细胞处理以DPI可引起剂量依赖性和时间依赖性的生长抑制，诱导DNA断裂和促凋亡因子Bax的蛋白水平。除此之外，DPI还可显著地诱导p53的表达和其磷酸化，在DNA损伤或无法修复的DNA周期阻滞中，诱导促凋亡基因的表达。
体内研究

Intraplantar injection of 2 mM Diphenyleneiodonium chloride to the hindpaw causes licking or biting behavior. Diphenyleneiodonium chloride treatment immediately or 24 h after lipopolysaccharide (LPS) injection significantly attenuates the LPS-induced loss of O4 positive cells. Treatment with Diphenyleneiodonium chloride either immediately or 24 h after LPS injection significantly ameliorates the LPS-induced disorganization of the white matter nerve fibers. However, treatment with DPI 48 h after LPS injection does not appear to correct the LPS-induced white matter damage. DPI treatment either immediately or 24 h after LPS injection significantly reduces the accumulation of both gp91phox and p67phox in the membrane fraction.