SEC

生物活性体外研究体内研究 SEC 试剂级价格

SEC

CAS号:1802997-81-4
英文名:1H-Pyrazole-5-carboxylic acid, 1-[(2S)-3-(4-chlorophenoxy)-2-hydroxypropyl]-3-(4-methoxyphenyl)-, ethyl ester
中文名:SEC
CBNumber:CB68108739
分子式:C22H23ClN2O5
分子量:430.88
MOL File:1802997-81-4.mol

SEC化学性质

沸点 :627.7±55.0 °C(Predicted)
密度 :1.27±0.1 g/cm3(Predicted)
酸度系数(pKa) :13.31±0.20(Predicted)
形态 :Oil
颜色 :Light yellow to yellow

SEC性质、用途与生产工艺

生物活性 SEC 通过 AMPK/mTORC1/STAT3 信号通路诱导 ANXA7 GTPase 的激活。SEC 选择性的促进癌细胞的凋亡，诱导 ITGB4 核转位，表达高水平 ITGB4。

体外研究

SEC (20 µM) inhibits the cell migration of HEK 293T RKIP ^−/− cells and PC3 prostate cancer cells.
SEC (20 µM) significantly increases AMPK phosphorylation in PC3 cells, which is inverted by ANXA7 GTPase specific inhibitor ABO, indicating that activated ANXA7 with enhanced phosphorylation level promotes AMPK phosphorylation ( cell selectively ).

Cell Viability Assay

Cell Line:	HEK293T RKIP ^−/− cells. ^[1]
Concentration:	20 µM.
Incubation Time:	24 h.
Result:	Suppressed HEK293T RKIP ^−/− cell migration while had no effect on HEK293T RKIP ^+/+ cells.

体内研究

SEC (3 mg/kg/day or 18 mg/kg/day) suppresses metastasis in PC-3M-Luc orthotopic implantation nude mice model.

Animal Model:	Luciferase-labeled PC-3M-Luc cells (2×10 ⁶ per 50 µL sterile HBSS ^−/− ) are orthotopically inoculated into the prostates of 8-week-old nude mice.
Dosage:	3 mg/kg/day or 18 mg/kg/day.
Administration:	IP daily for 3 weeks.
Result:	Suppressed the lymph node metastatic capacities of PC-3M-Luc cells in the nude mice model. Decreased the mRNA level of CCL2 , APLN and IL6ST in implanted tumors. Did not affect the body weight of the mice.