菝葜

生物活性靶点体外研究体内研究菝葜试剂级价格

菝葜

CAS号:126-18-1
英文名:SMILAGENIN
中文名:菝葜
CBNumber:CB6750868
分子式:C27H44O3
分子量:416.64
MOL File:126-18-1.mol

菝葜化学性质

熔点 :~185 °C
比旋光度 :D25 -69° (c = 0.5 in chloroform)
沸点 :474.91°C (rough estimate)
密度 :1.0362 (rough estimate)
折射率 :1.4700 (estimate)
储存条件 :Store at -20°C
溶解度 :Ethanol: ≥ 10 mg/mL (24.00 mM); DMSO: < 1 mg/mL (insoluble or slightly soluble)
酸度系数(pKa) :15.14±0.70(Predicted)
形态 :Solid
颜色 :White to off-white

安全信息

WGK Germany :3
F :10

菝葜化学药品说明书

菝葜|药典2005版

菝葜性质、用途与生产工艺

生物活性 Smilagenin (SMI) 是一种脂溶性小分子甾体皂苷元，来自知母 Rhizoma anemarrhenae 和天竺葵 Radix asparagi，广泛用于慢性神经退行性疾病的研究。Smilagenin (SMI) 通过增加毒蕈碱受体亚型 1 (M1)-受体的的密度来改善老年大鼠的记忆。 Smilagenin (SMI) 通过刺激脑源性神经营养因子的基因表达减弱 Aβ (25-35) 诱导的神经变性，可用于 AD 的研究。
靶点

Human Endogenous Metabolite

体外研究

Smilagenin (10 μM; 24 hours) increases SH-SY5Y cell survival compared with Aβ(25-35) intoxicated cells. Smilagenin (10 μM; 24 hours) increases neurotrophic factor (GDNF) and neurotrophic factor (BDNF) mRNA level by promoting CREB phosphorylation in 1-methyl-4-phenylpyridimium (MPP ⁺ ) treated SH-SY5Y cells.

Cell Viability Assay

Cell Line:	SH-SY5Y cells
Concentration:	10 μM
Incubation Time:	24 hours
Result:	Elevated the SH-SY5Y cell viability.

RT-PCR

Cell Line:	SH-SY5Y cells
Concentration:	10 μM
Incubation Time:	24 hours
Result:	Increased GDNF and BDNF transcription.

体内研究

Smilagenin (intragastric administration; 10 or 26 mg/kg, once daily; 60 days) prevents the impairment of dopaminergic neurons in chronic MPTP/probenecid-induced mouse model.

Animal Model:	MPTP/probenecid-induced mouse model
Dosage:	10 or 26 mg/kg
Administration:	Intragastric administration; 10 or 26 mg/kg; once daily; 60 days
Result:	Ameliorated locomotor ability of MPTP/probenecid-lesioned mice.