盐酸石蒜碱

生物活性靶点体外研究体内研究盐酸石蒜碱试剂级价格

盐酸石蒜碱

CAS号:2188-68-3
英文名:Lycorine hydrochloride
中文名:盐酸石蒜碱
CBNumber:CB6415905
分子式:C16H17NO4
分子量:287.31
MOL File:2188-68-3.mol

盐酸石蒜碱化学性质

熔点 :206 °C
储存条件 :Keep in dark place,Inert atmosphere,2-8°C
溶解度 :Soluble in water (15 mg/ml)
形态 :powder
颜色 :White to off-white
稳定性 :Stable for 2 years from date of purchase as supplied. Solutions in water may be stored at -20°C for up to 1 month.

安全信息

危险品标志 :T
危险类别码 :25
安全说明 :45
危险品运输编号 :UN 2811 6.1/PG 3
WGK Germany :3

盐酸石蒜碱性质、用途与生产工艺

生物活性 Lycorine hydrochloride 是石蒜的主要活性成分，也是黑色素瘤血管生成抑制剂，具有抗肿瘤活性。Lycorine hydrochloride 能有效抑制 Hey1B 细胞的有丝分裂增殖 (IC50 为 1.2 μM)。
靶点

IC50: 1.2 μM (Hey1B cells )

体外研究

Lycorine hydrochloride (0-25 μM; 48 hours; C8161 cells) treatment markedly suppresses the expression of vascular endothelial (VE)-cadherin in a dose-dependent manner and also slightly diminishes the expression of Sema4D in C8161 cells. However, the expression of the other six genes is not affected.
Lycorine hydrochloride (0-25 μM; 48 hours; C8161 cells) treatment markedly reduces VE-cadherin protein levels in C8161 cells in a dose-dependent fashion.

RT-PCR

Cell Line:	C8161 cells
Concentration:	0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time:	48 hours
Result:	Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells.

Western Blot Analysis

Cell Line:	C8161 cells
Concentration:	0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM
Incubation Time:	48 hours
Result:	Markedly reduced VE-cadherin protein levels in C8161 cells in a dose-dependent fashion.

体内研究

Lycorine hydrochloride (0-15 μg; subcutaneous injection; for 14 days; BALB/C nude mice) treatment has an inhibitory effect on melanoma cell-mediated tumor vasculogenic mimicry.

Animal Model:	6- to 8-week-old BALB/C nude mice
Dosage:	0 μg, 7.5 μg, 15 μg
Administration:	Subcutaneous injection; for 14 days
Result:	Showed an inhibitory effect on melanoma cell-mediated tumor vasculogenic mimicry.