己酮可可碱

生物活性靶点体外研究体内研究己酮可可碱试剂级价格

己酮可可碱

CAS号:6493-05-6
英文名:Pentoxifylline
中文名:己酮可可碱
CBNumber:CB5721652
分子式:C13H18N4O3
分子量:278.31
MOL File:6493-05-6.mol

己酮可可碱化学性质

熔点 :98-100°C
沸点 :421.13°C (rough estimate)
密度 :1.1713 (rough estimate)
折射率 :1.6000 (estimate)
储存条件 :Sealed in dry,Room Temperature
溶解度 :H₂O: ≥43 mg/mL
形态 :solid
酸度系数(pKa) :0.50±0.70(Predicted)
颜色 :white
最大波长(λmax) :276nm(lit.)
Merck :14,7136
CAS 数据库 :6493-05-6(CAS DataBase Reference)
NIST化学物质信息 :Pentoxifylline(6493-05-6)
EPA化学物质信息 :1H-Purine-2,6-dione, 3,7-dihydro-3,7-dimethyl-1-(5-oxohexyl)- (6493-05-6)

安全信息

危险品标志 :Xn
危险类别码 :22
安全说明 :36
WGK Germany :3
RTECS号 :XH2475000
海关编码 :2939590000
毒性 :LD50 orally in mice: 1385 mg/kg (Popendiker)

己酮可可碱化学药品说明书

己酮可可碱肠溶片|药典2005版
己酮可可碱|药典2005版

己酮可可碱性质、用途与生产工艺

生物活性 Pentoxifylline (BL-191) 是一种血液流变学改善剂。Pentoxifylline 是一种具有口服活性的，非选择性的磷酸二酯酶 (PDE) 抑制剂，具有免疫调节、抗炎、血液流变学改善、抗纤溶和抗增殖作用。Pentoxifylline 可用于研究周围血管疾病、脑血管疾病和其他一些涉及局部微循环缺陷的疾病。
靶点

PDE

体外研究

Pentoxifylline (0.1-50 mM; 24-48 hours) inhibits cell proliferation in a dose-dependent manner.
Pentoxifylline (0.5 mM; 12-36 hours) increases apoptosis and decreases autophagy levels in MDA-MB-231 cells.
Pentoxifylline (0.5 mM; 12-36 hours) induces autophagy in MDA-MB-231 cells.
Pentoxifylline (0.5 mM; 24-48 hours) blocks cell cycle at the G0/G1 phase.
Pentoxifylline results in high LC3-II/LC3-ratio.

Cell Autophagy Assay

Cell Line:	MDA-MB-231 cells
Concentration:	0.1 mM, 1 mM, 5 mM , 10 mM, 50 mM
Incubation Time:	24 hours, 48 hours
Result:	Inhibited cell proliferation in a dose-dependent manner.

Apoptosis Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	0.5 mM
Incubation Time:	12 hours, 24 hours, 36 hours
Result:	Induced apoptosis.

Apoptosis Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	0.5 mM
Incubation Time:	24 hours, 48 hours
Result:	Induced approximately 20-28% of cell autophagy.

Cell Cycle Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	0.5 mM
Incubation Time:	24 hours, 48 hours
Result:	Induced G0/G1 phase arrest.

Western Blot Analysis

Cell Line:	MDA-MB-231 cells
Concentration:	0.5 mM
Incubation Time:	24 hours, 48 hours
Result:	Induced high LC3-II/LC3-ratio.

体内研究

Pentoxifylline (200 mg/kg; i.p.) has a protective effect on rats with transient global ischemia and could reduce cognitive impairment.

Animal Model:	Adult male Wistar rats 12-13-weeks-old (250-300 g)
Dosage:	200 mg/kg
Administration:	Intraperitoneal injection, at 1hr before and 3 hr after ischemia
Result:	Significantly improved the spatial memory and effects were significant different from those of sham-operated and vehicle groups.

化学性质白色针状结晶。熔点105℃。易溶于水、苯、乙醇、氯仿，微溶于乙醇。无臭，味苦。
用途该品为血管扩张药，能改善脑和四肢的血液循环。小鼠口服LD50为1385mg/kg。
生产方法由1，3-溴氯丙烷与乙酰乙酸乙酯在80℃左右环合得到2-甲基-3-乙氧羰基-5，6-二氢吡喃，再将其开环、溴化生成1-溴-5-已酮，然后与可可碱钠盐缩合制成已酮可可碱。