ANTI-TYROSINE HYDROXYLASE

Rabbit

适用于: WB, Flow Cyt, IHC-P, ICC/IF

与反应: Mouse, Rat, Human 预测可用于: Pig

Synthetic peptide within Human Tyrosine Hydroxylase aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.

Immunocytochemistry/ Immunofluorescence - Anti-Tyrosine Hydroxylase antibody (ab137869)

Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor cell line) cells labeling Tyrosine Hydroxylase with Purified ab137869 at 1:100 dilution (5.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

酪氨酸羟化酶（Tyrosine hydroxylase）是负责催化氨基酸L-酪氨酸转变为二羟基苯丙氨酸（多巴）的酶。因此它使用四氢生物蝶呤作为辅酶。多巴是多巴胺的一个前体，相应地，后者亦是去甲肾上腺素与肾上腺素的前体。在人体中，酪氨酸羟化酶由TH基因编码出来。
酪氨酸羟化酶可以被药物α-甲基-对-酪氨酸（甲基酪氨酸）所抑制。此抑制作用可以导致脑部多巴胺与去甲肾上腺素的消耗，这是因为缺乏前体L-多巴（L-3,4-二羟基苯丙氨酸），此物质可以由酪氨酸羟化酶所合成。此药很少被使用并会导致抑郁，但它在治疗嗜铬细胞瘤以及抗高血压方面很有用处。

双抗体夹心法测定标本中Tyrosine Hydroxylase水平。用纯化的Tyrosine Hydroxylase抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Tyrosine Hydroxylase，再与HRP标记的Tyrosine Hydroxylase抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的Tyrosine Hydroxylase呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中Tyrosine Hydroxylase浓度。