甘草西定

功效作用生物活性体外研究体内研究甘草西定试剂级价格

甘草西定

CAS号:30508-27-1
英文名:licoricidin
中文名:甘草西定
CBNumber:CB51380788
分子式:C26H32O5
分子量:424.53
MOL File:30508-27-1.mol

甘草西定化学性质

熔点 :161.0-162.5℃
沸点 :610.8±55.0 °C(Predicted)
密度 :1.171±0.06 g/cm3(Predicted)
储存条件 :Store at -20°C
溶解度 :Soluble in DMSO
形态 :Solid
酸度系数(pKa) :10.10±0.40(Predicted)
颜色 :White to off-white

甘草西定性质、用途与生产工艺

功效作用研究发现，甘草西定对耐甲氧西林金黄色葡萄球菌等耐药菌以及多种口腔致病菌具有抗菌活性，在肿瘤的防治方面也发挥了积极的作用。此外，甘草西定还具有抗炎、调节免疫、抗氧化、神经保护等药理活性。
生物活性 Licoricidin (LCD) 从甘草 Glycyrrhiza uralensis Fisch 中分离，具有抗癌活性。Licoricidin (LCD) 通过诱导周期停滞，诱导细胞凋亡 (apoptosis) 和自噬 (autophagy)，可用于结直肠癌的研究。Licoricidin (LCD) 通过抑制肿瘤血管生成和淋巴管生成以及肿瘤组织局部微环境的变化抑制肺转移。Licoricidin (LCD) 通过体外和体内 Akt 和 NF-κB 途径的失活，增强吉西他滨诱导的骨肉瘤 (OS) 细胞的细胞毒性。Licoricidin (LCD) 通过 ROS 清除阻断 UVA 诱导的光老化，限制 MMP-1 的活性，被认为是新的局部应用的抗衰老制剂中的活性成分。

体外研究

Licoricidin (LCD) (0-20 μM; 24 hours) dose-dependently inhibits the viability of colon cancer cell lines with various pathological and genetic characters, namely SW480, HCT116, SW620 and LoVo cells, with IC ₅₀ values of 7.2, 5.4, 4.5 and 5.1 μM, respectively. Licoricidin (LCD) (0-20 μM; 0-12 hours) induces cell apoptosis was accompanied with the activation of caspase-3 by cleavage in a time- and dose-dependent manner. Licoricidin (LCD) (0-20 μM; 0-12 hours) induces autophagy of SW480 cells, increases the cleavage of LC3-I to LC3-II and the degradation of p62 in a time and dose dependent manner. Licoricidin (LCD) (0-5 μg/ml; 18 hours) inhibits cell migration, MMP-9 secretion, and VCAM expression in 4T1 cells.

Cell Viability Assay

Cell Line:	SW480, HCT116, SW620 and LoVo cells
Concentration:	0-20 μM
Incubation Time:	24 hours
Result:	Decreased colon cancer cell lines viability.

Western Blot Analysis

Cell Line:	SW480 cells
Concentration:	0 μM, 2.5 μM, 5 μM, 10 μM, 20 μM
Incubation Time:	0 hours, 1 hour, 3 hours, 6 hours, 12 hours
Result:	Induced cell apoptosis.

体内研究

Licoricidin (LCD) (intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 15 days) significantly inhibited the growth of SW480 xenografts in nude mice with an inhibitory rate of 43.5%. Licoricidin (LCD) (intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 32 days) reduces pulmonary metastasis and the expression of CD45, CD31, HIF-1α, iNOS, COX-2, and VEGF-A in tumor tissues, additionally, decreases protein expression of VEGF-R2, VEGF-C, VEGF-R3, and LYVE-1 in tumor tissues of licoricidin-treated mice.

Animal Model:	SW480 xenografted tumor growth in nude mice
Dosage:	5, 10, or 20 mg/kg
Administration:	Intraperitoneal injection; once daily; 15 days
Result:	Decreased tumor volumes.

Animal Model:	BALB/c mouse orthotopic model
Dosage:	5, 10, or 20 mg/kg
Administration:	Intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 32 days
Result:	Inhibited Lung Metastasis of 4T1 Murine Mammary Carcinoma cells.