新霉素 B 硫酸盐

应用生物活性靶点体外研究新霉素 B 硫酸盐试剂级价格

新霉素 B 硫酸盐

CAS号:4146-30-9
英文名:Framycetin sulphate
中文名:新霉素 B 硫酸盐
CBNumber:CB4921057
分子式:C23H48N6O17S
分子量:712.72
MOL File:4146-30-9.mol

新霉素 B 硫酸盐化学性质

熔点 :>160°C (dec.)
储存条件 :Hygroscopic, -20°C Freezer, Under inert atmosphere
溶解度 :Freely soluble in water, very slightly soluble in alcohol, practically insoluble in acetone.
形态 :Solid
颜色 :White to Off-White
水溶解性 :Water : 250 mg/mL (275.06 mM; Need ultrasonic)
稳定性 :Hygroscopic
CAS 数据库 :4146-30-9(CAS DataBase Reference)

新霉素 B 硫酸盐性质、用途与生产工艺

应用
新霉素 B 硫酸盐的抗菌谱及广，对革兰氏阴性菌和革兰氏阳性菌有极好的杀灭作用，广泛应用于医药和兽药领域。
生物活性 Framycetin sulfate (Neomycin B sulfate) 是一种氨基糖苷类抗生素，是一种有效的 RNase P 裂解活性抑制剂，Ki 为 35 μM。Framycetin sulfate 竞争 RNase P RNA 中特定的二价金属离子结合位点。Framycetin sulfate 抑制锤头状核酶 (hammerhead ribozyme)，Ki 值为 13.5 μM。Framycetin sulfate 是 5″-azido neomycin B 前体，与 miR-525 中的 Drosha 位点结合，可用于肝性脑病和肠致病性大肠杆菌感染。
靶点

Ki: 35 μM (RNase P cleavage activity) and 13.5 μM (hammerhead ribozyme)
体外研究

The inhibition of RNase P RNA cleavage by Framycetin sulfate (Neomycin Bsulfate; Fradiomycin Bsulfate) is sensitive to pH and an increase in pH suppresses the inhibition in other systems.
Framycetin sulfate targets the bacterial and human ribosome and affect translation. 5″-azido neomycin B and Framycetin sulfate selectively inhibit production of the mature miRNA, boosts a downstream protein, and inhibits invasion in HCC cell line.
Framycetin sulfate binds to a structural rather than a sequence motif of the RNA. Its primary cognate target is the decoding site of the 16S rRNA, but it also binds to the Rev-responsive element in HIV-1, group I introns, and the hammerhead ribozyme, and thus inhibits their biological function.
Framycetin sulfate induces misreading of the genetic code during translation and inhibits several ribozymes. The ribosomal target site is the 16 S rRNA 1400 to 1500 region.