衣霉素

生物活性体外研究体内研究衣霉素试剂级价格

衣霉素

CAS号:11089-65-9
英文名:TUNICAMYCIN
中文名:衣霉素
CBNumber:CB4211576
分子式:C37H60N4O16
分子量:816.89
MOL File:11089-65-9.mol

衣霉素化学性质

熔点 :234-235℃ (decomposition)
比旋光度 :D20 +52° (c = 0.5 in pyridine)
闪点 :87℃
储存条件 :2-8°C
溶解度 :Soluble in methanol, ethanol, DMSO, or DMF
形态 :White to off-white solid
颜色 :White
敏感性 :Moisture & Light Sensitive
BRN :6888090
稳定性 :Stable for 2 years from date of purchase as supplied. Solutions in DMSO or ethanol may be stored at -20°C for up to 1 month.
InChIKey :YJQCOFNZVFGCAF-WPTOCQRYSA-N
EPA化学物质信息 :Tunicamycin (11089-65-9)

安全信息

危险品标志 :T+,T
危险类别码 :28
安全说明 :28-37/39-45
危险品运输编号 :UN 3462 6.1/PG 2
WGK Germany :3
RTECS号 :YO7980200
F :8-10-18-21
危险等级 :6.1(a)
包装类别 :II
海关编码 :29419090

衣霉素性质、用途与生产工艺

生物活性 Tunicamycin 是一种同源核苷抗生素的混合物, 可抑制 N-糖基化并阻断 GlcNAc 磷酸转移酶 (GPT)。Tunicamycin 引起细胞内质网 (ER) 中未折叠蛋白的积累并诱导 ER 应激，并导致 DNA 合成受阻和 G1 期细胞周期停滞。Tunicamycin 可抑制革兰氏阳性细菌，酵母，真菌和病毒，并具有抗癌活性。Tunicamycin 增加宫颈癌细胞中外泌体的释放。

体外研究

Tunicamycin (2 µg/mL; 24 hours; CD44+/CD24- and original MCF7 cells) treatment increases the spliced XBP-1, ATF6 nuclear translocation level and CHOP protein expression in CD44+/CD24- and original MCF7 cells.
Tunicamycin-induced ER stress suppresses CD44+/CD24- phenotype cell subpopulation and in vitro invasion and accelerates tumorosphore formation. Under effect of Tunicamycin, the results show that inhibited invasion, increased cell death, suppressed proliferation and reduced migration in the CD44+/CD24- and CD44+/CD24- rich MCF7 cell culture.

Western Blot Analysis

Cell Line:	CD44+/CD24- and original MCF7 cells ^[1]
Concentration:	2 µg/mL
Incubation Time:	24 hours
Result:	Increased level of spliced XBP-1, ATF6 nuclear translocation and CHOP protein expression are detected in CD44+/CD24- and original MCF7 cells.

体内研究

Tunicamycin (0.1 mg/kg or 0.5 mg/kg) treatment dramatically suppresses tumor growth in the CD133 ^+/- MHCC97L cells xenograft model (BALB/c ( nu/nu ) mice).