2-3-(4-苯甲酰苯甲酰)腺苷 5-三磷酸三乙铵盐(-20°C)

生物活性靶点体外研究体内研究 2-3-(4-苯甲酰苯甲酰)腺苷 5-三磷酸三乙铵盐(-20°C) 试剂级价格

CAS号:112898-15-4
英文名:2'- AND 3'-O-(4-BENZOYLBENZOYL)-ADENOSINE 5'-TRIPHOSPHATE TRIETHYLAMMONIUM SALT
中文名:2-3-(4-苯甲酰苯甲酰)腺苷 5-三磷酸三乙铵盐(-20°C)
CBNumber:CB2746238
分子式:C30H39N6O15P3
分子量:816.58
MOL File:112898-15-4.mol

2-3-(4-苯甲酰苯甲酰)腺苷 5-三磷酸三乙铵盐(-20°C)化学性质

储存条件 :-20°C
溶解度 :≤14 mg/ml in DMSO
形态 :solid
颜色 :white to off-white
水溶解性 :Soluble in water (supplied pre-dissolved at a concentration of 5mM)
InChIKey :HVOVBTNCGADRTH-WBLDMZOZSA-N

安全信息

WGK Germany :3

2-3-(4-苯甲酰苯甲酰)腺苷 5-三磷酸三乙铵盐(-20°C)性质、用途与生产工艺

生物活性 BzATP triethylammonium salt 是一种 P2X 受体激动剂，对 P2X1，P2X2，P2X3，P2X2/3，P2X4 和 P2X7 的 pEC50 分别为 8.74，5.26，7.10，7.50， 6.19，6.31 和 5.33。BzATP triethylammonium salt 对 P2X7 受体有效，对大鼠 P2X7 和小鼠 P2X7 的EC50 分别为 3.6 μM 和 285 μM。
靶点

pEC50: 8.74 (P2X1), 5.26 (P2X2), 7.10 (P2X3), 6.19 (P2X2/3), 6.31 (P2X4), 5.33 (P2X7)
EC50 3.6 μM (rat P2X7); 285 μM (mouse P2X7)

体外研究

BzATP (10-1000 μM; 24 h) promotes the proliferation and migration of U87 and U251 glioma cells.
P2X7R protein expression is induced by BzATP (100 μM; 6-48 h) in human glioma cells.

Cell Proliferation Assay

Cell Line:	U87 and U251 glioma cells
Concentration:	5, 10, 50, 100, 500 and 1000 μM
Incubation Time:	2, 6, 12, 24, 48 and 72 hours
Result:	The proliferation of U87 and U251 glioma cell lines was significantly increased in the presence of 10-1000 uM and 100-1000 μM, respectively. The peak of cell proliferation of both U87 and U251 cell lines was at 100 μM. The optimal incubation time is 24 hours in both U87 and U251 cells lines.

Western Blot Analysis

Cell Line:	U87 and U251 glioma cells
Concentration:	100 μM
Incubation Time:	6-48 hours
Result:	Induced the upregulation of P2X7R.

体内研究

BzATP (5 mg/kg) significantly promotes P2X7R expression in the intestines compared with intestines in the sham group and the control group after cecal ligation and puncture (CLP) induction.

Animal Model:	Male 2-month-old C57BL/6 mice (each weighing between 20 and 25 g)
Dosage:	5 mg/kg
Administration:	Injected through the intraperitoneal route
Result:	At 48 hours, mice in the treated group and control group exhibited mortalities of 91% and 86%, respectively.