N-辛酰基-D-神经鞘氨醇

生物活性靶点体外研究体内研究 N-辛酰基-D-神经鞘氨醇试剂级价格

N-辛酰基-D-神经鞘氨醇

CAS号:74713-59-0
英文名:C8 CERAMIDE
中文名:N-辛酰基-D-神经鞘氨醇
CBNumber:CB2432038
分子式:C26H51NO3
分子量:425.69
MOL File:74713-59-0.mol

N-辛酰基-D-神经鞘氨醇化学性质

熔点 :68-70°C
沸点 :541.37°C (rough estimate)
密度 :0.9974 (rough estimate)
折射率 :1.6000 (estimate)
储存条件 :−20°C
溶解度 :DMSO or ethanol: soluble
形态 :waxy solid
颜色 :White
稳定性 :Temperature Sensitive
CAS 数据库 :74713-59-0(CAS DataBase Reference)

安全信息

安全说明 :24/25
WGK Germany :3
海关编码 :29225000

N-辛酰基-D-神经鞘氨醇性质、用途与生产工艺

生物活性 C8-Ceramide (N-Octanoyl-D-erythro-sphingosine) 是一种细胞渗透性的内源性神经酰胺类似物。C8-Ceramide 具有具有抗增殖特性，能作为化疗试剂。C8-Ceramide 能刺激树突状细胞促进 T 细胞对病毒感染的反应。C8-Ceramide 在体外能轻微地诱导 PKC 活化。
靶点

PKC

apoptosis

autophagy

体外研究

C8-ceramide (3 μM; 48 hours) irreversibly reduces tumor-cell proliferation and induces morphological changes.
C8-ceramide can induce necrosis-like cell death, but does not induce caspase-dependent cleavage of PARP (biochemical marker of apoptosis) in human cervical tumor cells.
C8-ceramide may increase the endogenous ROS level (10-30 µM; 24 hours) by regulating the switch of SOD1 and SOD2, causing the anti-proliferation (10-50 µM; 24 hours), and consequently triggering the apoptosis (10-50 µM; 48 hours) of NSCLC H1299 cells.

Cell Viability Assay

Cell Line:	CALO cells, INBL cells, HeLa cells
Concentration:	3 μM
Incubation Time:	48 hours
Result:	Markedly reduced the tumor cell number.

Cell Proliferation Assay

Cell Line:	H1299 cells
Concentration:	10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time:	24 hours
Result:	Decreased the rate of cellular proliferation in a dose-dependent manner, with an IC ₅₀ of 22.9 µM.

Cell Cycle Analysis

Cell Line:	H1299 cells
Concentration:	10 µM, 20 µM, 30 µM, 40 µM, 50 µM
Incubation Time:	24 hours
Result:	Caused the G1 arrest.

Apoptosis Analysis

Cell Line:	H1299 cells
Concentration:	10 µM, 20 µM, 30 µM
Incubation Time:	24 hours, 48 hours
Result:	Increased the level of cleaved caspase-3.

体内研究

C8-ceramide (0.1 mg/kg; intranasal administration) induces more robust CD8 + and CD4 + T cell responses to viral infections in virus infected mice.

Animal Model:	C57BL/6 mice, with lymphocytic choriomeningitis virus infected
Dosage:	0.1 mg/kg
Administration:	Intranasal administration
Result:	Increased the CD8 ⁺ T cell response to influenza in the lungs.