五味子丙素

生物活性体外研究体内研究

五味子丙素

CAS号:61301-33-5
英文名:WUWEIZISU C
中文名:五味子丙素
CBNumber:CB2327435
分子式:C22H24O6
分子量:384.42
MOL File:61301-33-5.mol

五味子丙素化学性质

熔点 :122-123℃
沸点 :549.2±50.0 °C(Predicted)
密度 :1.232
溶解度 :DMSO : 8.33 mg/mL (21.67 mM; Need ultrasonic)H2O : < 0.1 mg/mL (insoluble)
形态 :powder
颜色 :White
LogP :5.623 (est)

五味子丙素性质、用途与生产工艺

生物活性 Schisandrin C (Schizandrin-C) 是从五味子中分离得到的一种植物化学木脂素 (lignan)。Schisandrin C 具有多种生物活性，包括抗癌、抗炎和抗氧化作用。Schisandrin C 可用于癌症、阿尔茨海默病、肝病的研究。Schisandrin C 诱导细胞凋亡 (apoptosis)。

体外研究

Schisandrin C (25-100 μM; 48 hours) down-regulates expression levels of Bcl-2, Bcl-xL and a concomitant degradation of poly(ADP-ribose) polymerase (PARP). It also activates caspase-3 and -9 in U937 cells.Schisandrin C (25-100 μM; 48 hours) induces apoptosis in a dose-dependent manner. And the fragmentation of genomic DNA is also increased in U937 cells.Schisandrin C (25-100 μM; 72 hours) induces G1 arrest, it down-regulates cyclin D1, cyclin E, cyclin-dependent kinase (Cdk) 4 and E2Fs expression, and also exhibits inhibition of pRB, and up-regulation of the Cdk inhibitor p21(WAF1/CIP1).

Western Blot Analysis

Cell Line:	U937 cells
Concentration:	25 μM,50 μM,100 μM
Incubation Time:	48 hours
Result:	Decreased the expression of apoptosis related proteins.

Apoptosis Analysis

Cell Line:	U937 cells
Concentration:	25 μM,50 μM,100 μM
Incubation Time:	48 hours
Result:	Induced cell apoptosis.

Cell Cycle Analysis

Cell Line:	U937 cells
Concentration:	25 μM,50 μM,100 μM
Incubation Time:	48 hours
Result:	Induced growth inhibition and G1 arrest of U937 cells.

体内研究

Schisandrin C (lateral ventricle injection (i.c.v.); 15-150 μg/kg; 5 days) reduces Aβ1-42-induced memory deficits in the Y-maze test. Neurons in the hippocampus of SCH-C (15 μg/kg)-treated group returned to normal level, and and SCH-C group (150 μg/kg) has slight neuroprotective effects Aβ1-42-induced group. SCH-C (15 μg/kg) recoveres the activities of SOD and GSHPx and the ratios of GSH, decreases the levels of ChEtotal in the brain of the Aβ1–42-induced amnesic mice simultaneously.

Animal Model:	Aβ1-42-induced Alzheimer’s disease mice
Dosage:	15-150 μg/kg
Administration:	Lateral ventricle injection (i.c.v.); 15-150 μg/kg; 5 days
Result:	Exhibited potent neuroprotective effects linking to anti-ChEtotal activities and anti-oxidative mechanisms in Aβ1-42-induced amnestic mice.