Cilliobrevin D

生物活性靶点体外研究体内研究 Cilliobrevin D 试剂级价格

Cilliobrevin D

CAS号:1370554-01-0
英文名:Cilliobrevin D
中文名:Cilliobrevin D
CBNumber:CB22714664
分子式:C17H8Cl3N3O2
分子量:392.62
MOL File:1370554-01-0.mol

Cilliobrevin D化学性质

沸点 :591.8±50.0 °C(Predicted)
密度 :1.537±0.06 g/cm3(Predicted)
储存条件 :Store at -20°C
溶解度 :DMSO: 5 mg/mL (12.73 mM; ultrasonic and warming and heat to 80°C)
酸度系数(pKa) :5.99±0.20(Predicted)
形态 :Solid
颜色 :Light yellow to yellow

Cilliobrevin D性质、用途与生产工艺

生物活性 Ciliobrevin D (compound 5) 是一种具有细胞渗透性、可逆性和特异性的 AAA+ (ATPases associated with diverse cellular activities) ATPase 运动肌 cytoplasmic dynein 的拮抗剂。Ciliobrevin D 可干扰初级纤毛的形成并阻止 Hedgehog (Hh) 信号传导。
靶点
Target Value
cytoplasmic dynein
()
Hedgehog
()
体外研究

Cells treated with Ciliobrevin D exhibits abnormal (unfocused, multipolar, or collapsed) spindles with disrupted γ-tubulin localization in NIH-3T3 cells. Similar Ciliobrevin-induced spindle defects are observed in HeLa cells, although to a lesser extent. Ciliobrevin D addition also reversibly disrupts the pre-formed spindles of metaphase-arrested cells and reduces overall microtubule levels.
. Ciliobrevin D reversibly inhibits melanosome aggregation, but the non-cilia-disrupting derivative had no discernible effect at comparable doses. Ciliobrevin D similarly abrogates the movement of peroxisomes in Drosophila S2 cells.
体内研究

Knockdown of Dync1h1 or inactivation of dynein 1 by Ciliobrevin D in the testis in vivo perturbs spermatogenesis. Knockdown of Dync1h1 or the use of Ciliobrevin D to inactivate dynein 1 in the testis in vivo perturbs MT organization through changes in the spatial expression of EB1, perturbs F-actin organization, and perturbs distribution of adhesion protein complexes at the BTB, leading to a loss of BTB integrity. F-actin disorganization in the seminiferous epithelium following Dync1h1 knockdown or dynein 1 inactivation by Ciliobrevin D is mediated by changes in the spatiotemporal expression of actin regulatory proteins Arp3 and Eps8.