人参皂苷RK2
- CAS号:494753-69-4
- 英文名:Ginsenoside RK2
- 中文名:人参皂苷RK2
- CBNumber:CB22654117
- 分子式:C42H70O12
- 分子量:767
- MOL File:494753-69-4.mol
- 沸点 :854.5±65.0 °C(Predicted)
- 密度 :1.27±0.1 g/cm3(Predicted)
- 储存条件 :2-8°C
- 溶解度 :Soluble in methan
- 形态 :Powder
- 酸度系数(pKa) :12.85±0.70(Predicted)
- 颜色 :White to light yellow
人参皂苷RK2性质、用途与生产工艺
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用途
Rk2一般通过水解人参皂苷二醇组获得，但水解成分复杂，不利于分离并准确鉴定结构。本文通过酶法水解高纯度的人参皂苷Rk2，水解产物通过高分辨质谱和核磁确证。
- 功效与作用 人参皂苷Rk2能有效抑制肿瘤细胞生长，诱导分化癌细胞，具有抗浸润和抗转移疗效。
- 生物活性 Ginsenoside Rk1 人参皂苷 Rk1 是人参的高温加工提取物。 Ginsenoside Rk1 具有抗炎作用，抑制 Jak2/Stat3 信号通路和 NF-κB 的激活。 Ginsenoside Rk1 具有抗肿瘤作用，抗血小板聚集活性，抗炎作用，抗胰岛素抵抗，肾保护作用，抗菌作用，认知功能增强，脂质积聚减少和预防骨质疏松症。 Ginsenoside Rk1 通过触发细胞内活性氧 (ROS) 生成和阻断 PI3K/Akt 途径诱导细胞凋亡。
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体外研究
Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation.
RT-PCR
Cell Line: RAW264.7 cells Concentration: 10 μM, 20 μM, 40 μM Incubation Time: 6 hours Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells. Western Blot Analysis
Cell Line: RAW264.7 cells Concentration: 10 μM, 20 μM, 40 μM Incubation Time: 6 hours Result: Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells Cell Viability Assay
Cell Line: MDA-MB-231 cells Concentration: 0 μM, 40 μM, 80 μM, 120 μM Incubation Time: 48 hours Result: Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner. Cell Cycle Analysis
Cell Line: MDA-MB-231 cells Concentration: 0 μM, 40 μM, 80 μM, 120 μM Incubation Time: 24 hours Result: Induced G0/G1 phase arrest. Apoptosis Analysis
Cell Line: MDA-MB-231 cells Concentration: 0 μM, 40 μM, 80 μM, 120 μM Incubation Time: 24 hours Result: Induced apoptosis in MDA-MB-231 cells. - 化学性质 白色粉末，可溶于甲醇、乙醇、DMSO等有机溶剂，来源于人参。
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用途
用于含量测定/鉴定/药理实验等。
药理药效:抗癌、提高免疫功能、调节神经系统。 - 用途 人参皂苷Rk1是蒸参中提取的成分之一，具有抗肿瘤的生物活性。
- 公司名称：Carbosynth
- 联系电话：--
- 电子邮件：sales@carbosynth.com
- 国家：英国
- 产品数：6005
- 优势度：58
