人参皂苷RK2
人参皂苷RK2 性质
| 沸点 | 854.5±65.0 °C(Predicted) |
|---|---|
| 密度 | 1.27±0.1 g/cm3(Predicted) |
| 储存条件 | 2-8°C |
| 溶解度 | 溶于甲烷 |
| 形态 | 粉末 |
| 酸度系数(pKa) | 12.85±0.70(Predicted) |
人参皂苷RK2 用途与合成方法
Rk2一般通过水解人参皂苷二醇组获得，但水解成分复杂，不利于分离并准确鉴定结构。本文通过酶法水解高纯度的人参皂苷Rk2，水解产物通过高分辨质谱和核磁确证。
Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation.
RT-PCR
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells. |
Western Blot Analysis
| Cell Line: | RAW264.7 cells |
| Concentration: | 10 μM, 20 μM, 40 μM |
| Incubation Time: | 6 hours |
| Result: | Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells |
Cell Viability Assay
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 48 hours |
| Result: | Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner. |
Cell Cycle Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced G0/G1 phase arrest. |
Apoptosis Analysis
| Cell Line: | MDA-MB-231 cells |
| Concentration: | 0 μM, 40 μM, 80 μM, 120 μM |
| Incubation Time: | 24 hours |
| Result: | Induced apoptosis in MDA-MB-231 cells. |
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