人参皂苷RK2

用途功效与作用生物活性体外研究

人参皂苷RK2

CAS号:494753-69-4
英文名:Ginsenoside RK2
中文名:人参皂苷RK2
CBNumber:CB22654117
分子式:C42H70O12
分子量:767
MOL File:494753-69-4.mol

人参皂苷RK2化学性质

沸点 :854.5±65.0 °C(Predicted)
密度 :1.27±0.1 g/cm3(Predicted)
储存条件 :2-8°C
溶解度 :Soluble in methan
形态 :Powder
酸度系数(pKa) :12.85±0.70(Predicted)
颜色 :White to light yellow

人参皂苷RK2性质、用途与生产工艺

用途
Rk2一般通过水解人参皂苷二醇组获得，但水解成分复杂，不利于分离并准确鉴定结构。本文通过酶法水解高纯度的人参皂苷Rk2，水解产物通过高分辨质谱和核磁确证。
功效与作用人参皂苷Rk2能有效抑制肿瘤细胞生长，诱导分化癌细胞，具有抗浸润和抗转移疗效。
生物活性 Ginsenoside Rk1 人参皂苷 Rk1 是人参的高温加工提取物。 Ginsenoside Rk1 具有抗炎作用，抑制 Jak2/Stat3 信号通路和 NF-κB 的激活。 Ginsenoside Rk1 具有抗肿瘤作用，抗血小板聚集活性，抗炎作用，抗胰岛素抵抗，肾保护作用，抗菌作用，认知功能增强，脂质积聚减少和预防骨质疏松症。 Ginsenoside Rk1 通过触发细胞内活性氧 (ROS) 生成和阻断 PI3K/Akt 途径诱导细胞凋亡。

体外研究

Ginsenoside Rk1 (0-40 μM; 6 hours) inhibits MCP-1 and TNF-α mRNA induced by lipopolysaccharide (LPS), expression of IL-1β is inhibited at 40 μM. Ginsenoside Rk1 (0-40 μM; 24 hours) inhibits phosphorylation of JAK2 and STAT3 (Tyr705 and Ser727) in LPS-induced RAW264.7 cells in a dose-dependent manner. Ginsenoside Rk1 (0-160 μM; 48 hours) results in cell viability significant decrease 75.52 ± 2.51% (40 μM), 52.72 ± 2.54% (80 μM), 17.41 ± 2.94% (120 μM)and 12.63 ± 3.24% (160 μM) compared with control. Ginsenoside Rk1 (0-120 μM; 24 hours) increases G0/G1 phase proportion accompanied with S and G2/M phase proportion decrease in MDA-MB-231 cells. Ginsenoside Rk1 (0-120 μM; 24 hours) promotes the percentage of apoptotic cells in a dose-dependent manner, exhibits to reduction of cell number with nucleus fragmentation, condensation and apoptotic body formation.

RT-PCR

Cell Line:	RAW264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:	6 hours
Result:	Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells.

Western Blot Analysis

Cell Line:	RAW264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:	6 hours
Result:	Inhibited JAK2-dependent STAT3 activation in LPS-activated RAW264.7 cells

Cell Viability Assay

Cell Line:	MDA-MB-231 cells
Concentration:	0 μM, 40 μM, 80 μM, 120 μM
Incubation Time:	48 hours
Result:	Inhibited MDA-MB-231 cells proliferation in a dose- and time-dependent manner.