5-(N-乙基-N-异丙基)阿米洛利

IC50: 10.5 μM (TRPP3 channel)
NHE
Macropinocytosis

In the presence of 100 μM EIPA, 10 μM benzamil, and 10 μM phenamil, ⁴⁵ Ca ²⁺ uptake decreases from 79±9 to 46±4 (58% remaining), 27±4 (34%), 29±5 (37%), and 38±4 (48%) pmol/oocyte/30 min (n=6, P=0.008), respectively. It is found that EIPA, benzamil, and phenamil rapidly and reversibly block Ca ²⁺ -activated TRPP3 channel activation at -50 mV, with IC ₅₀ s of 143±8 (n=36), 10.5±2.2 (n=28), 1.1±0.3 (n=30), and 0.14±0.04 μM (n=25), respectively. The number of autophagic vacuoles increases dramatically in the HAE and HPE groups after EIPA treatment compare with the HAN and HPN groups. EIPA regulates the initiation and maturation of the autophagy associated with amino acids in IEC-18 cells. In addition, the uptake of cinnamoylphenazine (CA-PZ) and neutral red (NR) is inhibited by EIPA.