洋橄榄叶素

概述用途生物活性靶点体外研究体内研究洋橄榄叶素试剂级价格

洋橄榄叶素

CAS号:37318-06-2
英文名:elaiophylin
中文名:洋橄榄叶素
CBNumber:CB11393845
分子式:C54H88O18
分子量:1025.27
MOL File:37318-06-2.mol

洋橄榄叶素化学性质

熔点 :179-182℃
沸点 :1080.5±65.0 °C(Predicted)
密度 :1.25
储存条件 :-20°C Freezer
溶解度 :DMSO (Slightly), Methanol (Slightly)
酸度系数(pKa) :12.18±0.70(Predicted)
形态 :White to off-white solid.
颜色 :White to Off-White

洋橄榄叶素性质、用途与生产工艺

概述盐霉素(Salinomycin)是由白色链霉菌经发酵产生的聚醚类动物专用抗生素，具有防治动物球虫病和促进畜禽生长发育的功能，被广泛地用于国内外的畜牧业中。但是，在盐霉素产品中，如果发酵工艺或菌种发生变化，将会有洋橄榄叶素的伴生，当含量超出一定限度，将对饲养对象产生不良反应，一定程度上会影响盐霉素的使用效果。
用途洋橄榄叶素是一种微生物代谢物，可用作对照品。
生物活性 Elaiophylin (Azalomycin B; Gopalamicin; Efomycin E) 是一种自噬 (autophagy) 抑制剂，在卵巢癌细胞中作为单一药剂发挥抗肿瘤活性。
靶点

Autophagy

体外研究

Elaiophylin-mediated autophagy inhibition and lysosomal dysfunction affect ovarian cancer cell survival during hypoxia. Exposure to Elaiophylin (0.025-0.5 μM; 24 hours) causes a significant increase in ovarian cancer SKOV3 cell death in hypoxia conditions.
In both the SKOV3 and A2780 cell lines, Elaiophylin (0.25, 0.5, 0.75 μM; 24 hours) treatment leads to significant activation of cleaved CASP9/caspase-9 and PARP1 and downregulation of BIRC5/survivin in a concentration-dependent manner.

Cell Viability Assay

Cell Line:	Ovarian cancer SKOV3 cells.
Concentration:	0.025, 0.05, 0.1, 0.2, 0.5 μM
Incubation Time:	24 hours
Result:	Caused a significant increase in ovarian cancer SKOV3 cells death in hypoxia conditions.

Western Blot Analysis

Cell Line:	Ovarian cancer SKOV3 cells; A2780 cells
Concentration:	0.25, 0.5, 0.75 μM
Incubation Time:	24 hours
Result:	Treatment led to significant activation of cleaved CASP9/caspase-9 and PARP1 and downregulation of BIRC5/survivin in a concentration-dependent manner.

体内研究

Treatment with 2 mg/kg Elaiophylin (given i.p. every 2 days for 21 days; in BALB/C athymic mice) significantly suppresses ovarian cancer SKOV3 cells growth compared with DMSO treatment, resulting in a 72% decrease in the average daily tumor growth rate compared with DMSO treatment .
Lower doses of Elaiophylin as a single agent exert significant antitumor activity, while higher doses lead to intestinal toxicity. Administration of a lower dose (2 mg/kg) of Elaiophylin as a single agent achieves a significant antitumor effect without toxicity in an orthotopic ovarian cancer model with metastasis. Toxic reactions are observed only in the 8 mg/kg group.

Animal Model:	4-wk-old BALB/C athymic mice with ovarian cancer SKOV3 cells
Dosage:	1 or 2 mg/kg
Administration:	Given i.p. every 2 days for 21 days
Result:	Treatment with 2 mg/kg significantly suppressed ovarian cancer SKOV3 cells growth compared with DMSO treatment.