CU-T12-9
- CAS号:1821387-73-8
- 英文名:CU-T12-9
- 中文名:CU-T12-9
- CBNumber:CB03146280
- 分子式:C17H13F3N4O2
- 分子量:362.31
- MOL File:1821387-73-8.mol
- 沸点 :524.3±50.0 °C(Predicted)
- 密度 :1.39±0.1 g/cm3(Predicted)
- 储存条件 :Store at -20°C
- 溶解度 :DMSO: 250 mg/mL (690.02 mM)
- 形态 :A solid
- 酸度系数(pKa) :3.53±0.10(Predicted)
- 颜色 :Light yellow to yellow
CU-T12-9性质、用途与生产工艺
- 生物活性 CU-T12-9 是一种特异性 TLR1/2 激动剂，EC50 为 52.9 nM。CU-T12-9 既激活先天免疫系统，又激活适应性免疫系统。CU-T12-9 选择性激活 TLR1/2 异二聚体而对 TLR2/6 没有作用。CU-T12-9 通过促进 TLR1 和 TLR2 二聚而激活 NF-κB 信号，致使下游 TNF-α，IL-10，和 iNOS 增加，从而提高身体的免疫反应。
-
靶点
TLR2
52.9 nM (EC 50 , in HEK-Blue cells)
TLR1
52.9 nM (EC 50 , in HEK-Blue cells)
-
体外研究
CU-T12-9 directly targets TLR1/2 to initiate downstream signaling. By binding to both TLR1 and TLR2, CU-T12-9 facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream signaling.
CU-T12-9 activates the TLR1/2 pathway by inducing NF-κB activation to trigger downstream signaling, such as secreted embryonic alkaline phosphatase (SEAP), NO, and TNF-α.
CU-T12-9 (0.39-100 μM; 24 hours) does not produce toxicity up to 100 μM in HEK-Blue hTLR2 and Raw 264.7 cells.
CU-T12-9 up-regulates the mRNA levels of TLR1, TLR2, TNF, IL-10, and iNOS. CU-T12-9 (0.1-10 μM) activates TLR1 mRNA and iNOS mRNA after Raw 264.7 cells are treated for 24 hours. CU-T12-9 (0.1-10 μM) activates TLR2 and IL-10 mRNA after Raw 264.7 cells are treated for 2 hours. CU-T12-9 (0.1-10 μM) activates TNF mRNA after Raw 264.7 cells are treated for 8 hours.Cell Cytotoxicity Assay
Cell Line: HEK-Blue hTLR2 and Raw 264.7 macrophage cells Concentration: 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100 μM Incubation Time: 24 hours Result: No toxicity was seen up to 100 μM. RT-PCR
Cell Line: Raw 264.7 cells Concentration: 0.1, 1, 10 μM Incubation Time: 24 hours for TLR1 and iNOS mRNA assay
2 hours for TLR2 and IL-10 mRNA assay
8 hours for TNF mRNA assay
Result: Triggered TLR1 mRNA and iNOS mRNA at 24 hours dose-dependently.
Dose-dependent activation of TLR2 mRNA and IL-10 mRNA at 2 hours.
Showed dose-dependent activation of TNF mRNA at 8 hours.
- 更新日期：2024/11/08
- 产品编号：HY-110353
- 产品名称：CU-T12-9
- CAS编号：
- 包装：1 mg
- 价格：522元
- 更新日期：2024/11/08
- 产品编号：HY-110353
- 产品名称：CU-T12-9 CU-T12-9
- CAS编号：1821387-73-8
- 包装：5mg
- 价格：1100元
- 公司名称：TargetMol Chemicals Inc.
- 联系电话：+1-781-999-5354 +1-00000000000
- 电子邮件：marketing@targetmol.com
- 国家：美国
- 产品数：32161
- 优势度：58
- 公司名称：Aladdin Scientific
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- 电子邮件：sales@aladdinsci.com
- 国家：美国
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- 公司名称：BOC Sciences
- 联系电话： 16314854226
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- 公司名称：MedChemExpress
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