CU-T12-9
CU-T12-9 性质
| 沸点 | 524.3±50.0 °C(Predicted) |
|---|---|
| 密度 | 1.39±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | 二甲基亚砜：250 mg/mL（690.02 mM） |
| 形态 | 固体 |
| 酸度系数(pKa) | 3.53±0.10(Predicted) |
| 颜色 | 浅黄至黄色 |
| InChI | 1S/C17H13F3N4O2/c1-21-14-7-6-13(24(25)26)8-16(14)23-9-15(22-10-23)11-2-4-12(5-3-11)17(18,19)20/h2-10,21H,1H3 |
| InChIKey | LITXVDAFEYLWQE-UHFFFAOYSA-N |
| SMILES | FC(F)(F)c1ccc(cc1)c2nc[n](c2)c3c(ccc(c3)[N+](=O)[O-])NC |
CU-T12-9 用途与合成方法
|
TLR2 52.9 nM (EC 50 , in HEK-Blue cells) |
TLR1 52.9 nM (EC 50 , in HEK-Blue cells) |
CU-T12-9 directly targets TLR1/2 to initiate downstream signaling. By binding to both TLR1 and TLR2, CU-T12-9 facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream signaling.
CU-T12-9 activates the TLR1/2 pathway by inducing NF-κB activation to trigger downstream signaling, such as secreted embryonic alkaline phosphatase (SEAP), NO, and TNF-α.
CU-T12-9 (0.39-100 μM; 24 hours) does not produce toxicity up to 100 μM in HEK-Blue hTLR2 and Raw 264.7 cells.
CU-T12-9 up-regulates the mRNA levels of TLR1, TLR2, TNF, IL-10, and iNOS. CU-T12-9 (0.1-10 μM) activates TLR1 mRNA and iNOS mRNA after Raw 264.7 cells are treated for 24 hours. CU-T12-9 (0.1-10 μM) activates TLR2 and IL-10 mRNA after Raw 264.7 cells are treated for 2 hours. CU-T12-9 (0.1-10 μM) activates TNF mRNA after Raw 264.7 cells are treated for 8 hours.
Cell Cytotoxicity Assay
| Cell Line: | HEK-Blue hTLR2 and Raw 264.7 macrophage cells |
| Concentration: | 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100 μM |
| Incubation Time: | 24 hours |
| Result: | No toxicity was seen up to 100 μM. |
RT-PCR
| Cell Line: | Raw 264.7 cells |
| Concentration: | 0.1, 1, 10 μM |
| Incubation Time: |
24 hours for TLR1 and iNOS mRNA assay
2 hours for TLR2 and IL-10 mRNA assay 8 hours for TNF mRNA assay |
| Result: |
Triggered TLR1 mRNA and iNOS mRNA at 24 hours dose-dependently.
Dose-dependent activation of TLR2 mRNA and IL-10 mRNA at 2 hours. Showed dose-dependent activation of TNF mRNA at 8 hours. |
CU-T12-9 价格(试剂级)
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2026-07-06 | S2979 | CU-T12-9 | 1821387-73-8 | 5mg | 2375.1 |
| 2026-07-06 | S2979 | 1821387-73-8 | 10mM (1mL in DMSO) | 3513.51 |
