476-28-8
基本信息
石蒜鹼
石蒜碱
石蒜碱标准品
石蒜碱盐酸盐
石蒜碱 HPL
石蒜碱(水仙碱)
石蒜提取物.石蒜碱
LYCORINE 石蒜碱
LYCORINE
Amaryline
Belamarine
amarylline
narcissine
NSC 401360
NSC 683873
LYCORINE HCL
(-)-LYCORINE
物理化学性质
| 外观性状 | 棱形大结晶。熔点275-280℃（分解）。溶于稀酸，难溶于醇、氯仿和石油醚，几乎水溶于水和碱类，对石蕊呈碱性反应。其盐酸盐为长针状结晶，熔点217℃（分解），带一分子结晶水的石蒜碱盐酸盐的熔点为206℃。可溶于20份水。 |
| 熔点 | 253-255℃ (dec.) |
| 比旋光度 | D16 -129° (c = 0.16 in 98% alc) |
| 沸点 | 429.61°C (rough estimate) |
| 密度 | 1.53 |
| 折射率 | 1.5500 (estimate) |
| 储存条件 | 2-8°C |
| 酸度系数(pKa) | 13.55±0.40(Predicted) |
| 形态 | powder |
安全数据
| 危险性符号(GHS) |  GHS06 |
| 警示词 | 危险 |
| 危险性描述 | H301 |
| 防范说明 | P264-P270-P301+P310-P321-P330-P405-P501 |
| 危险品标志 | T |
| 危险类别码 | 25 |
| 安全说明 | 45 |
| 危险品运输编号 | UN 2811 6.1/PG 3 |
| WGK Germany | 3 |
应用领域
制备方法
常见问题列表
Kd: 15.24 nM (SCAP)
Lycorine inhibits cell proliferation in a dose-dependent manner in the abovementioned 4 PCa cell lines, and the IC 50 ranged from 5 μM to 10 μM., it also shows Lycorine has little effects on PNT1A cell's proliferation.SCAP (SREBF chaperone) is an ER-toGolgi transport protein, undergoes a conformational change, and regulates/preserves SREBFs in the ER by forming a complex with INSIG1 (insulin induced gene 1).Lycorine (5-40 μM; 16 hours) significantly suppresses SREBFs activity (up to -70%) in a dosedependent manner and does not cause obvious cytotoxicity in cells.Lycorine (10-20 μM; 2-16 hours) dose- and time-dependently decreases the mature SREBF1 and SREBF2 proteins in HL-7702 cells.Lycorine (20 μM; 16 hours) neither activates NR1H3 transcription nor affect NR1H3 target genes, including ABCG5 and ABCG8, but Sterols activates NR1H3 transcription activity.Lycorine hydrochloride (0-25 μM; 48 hours) treatment markedly suppresses the expression of vascular endothelial (VE)-cadherin in a dose-dependent manner and also slightly diminishes the expression of Sema4D in C8161 cells. However, the expression of the other six genes is not affected.Lycorine hydrochloride (0-25 μM; 48 hours) treatment markedly reduces VE-cadherin protein levels in C8161 cells in a dose-dependent fashion.
Cell Viability Assay
| Cell Line: | HL-7702/SRE-Luc cells |
| Concentration: | 16 hours |
| Incubation Time: | 5 μM; 10 μM; 20 μM; 40 μM |
| Result: | Had no cytotoxicity on HL-7702 cells. |
Western Blot Analysis
| Cell Line: | HL-7702/SRE-Luc cells |
| Concentration: | 2 hours, 4 hours, 8 hours, 12 hours, 16 hours |
| Incubation Time: | 10 μM; 20 μM |
| Result: | Decreased p-SREBF1, m-SREBF1, p-SREBF2 and p-SREBF1 protein expression. |
RT-PCR
| Cell Line: | C8161 cells |
| Concentration: | 0 μM, 1.56 μM, 3.13 μM, 6.25 μM, 12.5 μM, 25 μM |
| Incubation Time: | 48 hours |
| Result: | Markedly suppressed the expression of VE-cadherin in a dose-dependent manner and also slightly diminished the expression of Sema4D in C8161 cells. |
Lycorine (oral chow; 15 mg/kg, 30 mg/kg; once daily) alleviates fat accumulation and metabolic syndrome, increases lipolysis and betaoxidation of fatty acid along with precursor and mature SREBFs in mice .
| Animal Model: | High-fat diet (HFD)-fed C57BL/6J mice |
| Dosage: | 15 mg/kg, 30 mg/kg |
| Administration: | Oral chow; once daily |
| Result: | Ameliorated high-fat diet-induced hyperlipidemia, hepatic steatosis, and insulin resistance in mice. |