98891-36-2

L-Homopropargyl glycine is a reactive methionine analog that contains an alkyne moiety. It is readily inserted into newly-synthesized proteins in place of methionine. L-Homopropargyl glycine can then be labeled or captured through click chemistry. This approach represents a fast, sensitive, and non-radioactive alternative to [35S]-methionine for the detection of nascent protein synthesis.

L-Homopropargylglycine (HPG) is a noncanonical amino acid analog of methionine that contains a terminal alkyne moiety. HPG-labeling is a fast, sensitive, non-toxic, and non-radioactive alternative to the traditional technique for detecting nascent protein synthesis.

HPG is the cell-permeable compound randomly incorporated into synthesizing protein instead of methionine during translation. The resulting alkyne-labeled full-length proteins can be detected via copper-catalyzed click reaction with fluorescent or biotin-labeled azides and used for subsequent microscopic imaging or purification tasks.

Alkyl-Chain