9004-02-8

Malic dehydrogenase catalyzes the dehydrogenation of L-malate by NAD+.

Purify the lipase by affinity chromatography on heparin-Sepharose; Ki 0.026mM for very low density lipoprotein. It is inhibited by 2-mercaptoethanol, Cys, Ca, Hg, Mg and Mn ions. Protamine sulfate, 1mg of bovine serum albumin/mL or in 50% glycerol at -70o, stabilises the lipase for several days. 60% loss of activity occurs at 0o/1hour in the presence of 1% of bovine serum albumin. [Shirai et al. Biochim Biophys Acta 665 504 1981.] Lipoproteins (from human plasma). Individual human plasma lipid peaks are removed from plasma by ultracentrifugation; then they are separated and purified by agarose-column chromatography. Fractions are characterised immunologically, chemically, electrophoretically and by electron microscopy. [Rudel et al. Biochem J 13 89 1974.]