ChemicalBook CAS DataBase List 9001-99-4

9001-99-4

Name	Ribonuclease A
CAS	9001-99-4
EINECS(EC#)	232-646-6
Molecular Formula	NULL
MDL Number	MFCD00132188

Chemical Properties

Appearance	Lyophilized
Melting point	243 °C (dec.)
alpha	D25 (per mg N) -0.47° (c = 5)
storage temp.	2-8°C
solubility	Reconstitute in 10mM acetate, pH 5.0 with or without 15mM calcium chloride
form	solid
color	white
PH	pH : 8.0
biological source	protein from bovine pancreas
Water Solubility	Soluble in water.
Sensitive	Moisture Sensitive
Specific Activity	85-140Kunitz units/mg protein
Major Application	diagnostic assay manufacturing
InChI	1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChIKey	CQOVPNPJLQNMDC-UHFFFAOYSA-N
SMILES	[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
EPA Substance Registry System	Nuclease, ribo-(9001-99-4)

Safety Data

Symbol(GHS)	GHS08
Signal word	Danger
Hazard statements	H334
Precautionary statements	P261-P284-P501
Safety Statements	22-24/25 less more
WGK Germany	3
RTECS	RF0760000
F	10-21
TSCA	Yes
HS Code	35079090
Storage Class	11 - Combustible Solids
Hazard Classifications	Resp. Sens. 1

Raw materials And Preparation Products

Hazard Information

Chemical Properties

Lyophilized

Uses

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA.Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.

General Description

Human chromosome 14q11.2 shows the presence of all human RNases. Only eight (RNase 1-8) of them were true called as canonical RNases. And the rest nine to thirteen are called as noncanonical RNases. Human RNase 1 is called as HP-RNase or human pancreatic RNase. Human RNase 2 is also called as EDN (eosinophils derived neurotoxin) that is isolated from eosinophils.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Purification Methods

Purify it by (NH4)2SO4 fractionation, followed by PC cellulose chromatography and affinity chromatography (using Sepharose 4B to which (G)n is covalently bonded). [Schmukler et al. J Biol Chem 250 2206 1975.] Ribozymes. These are ribonucleic acids which act like protein enzyme in catalyzing the making and breaking of peptide bonds as well as catalyzing reactions and cleavage of DNA and RNA molecules. The short RNAs are being intensively studied (see RNAi below). MicroRNAs (miRNA, see also below) are ubiquitous and are also genetically produced. They are involved in numerous reactions from splicing RNA, e.g. mRNA (messenger RNA) to controlling transcription of DNA to RNA, and translation of RNA to protein. Each miRNA is capable of being involved in a small number to hundreds of interactions with nucleic acids and with proteins.

Material Safety Data Sheet(MSDS)

Supplier

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Dalian Meilun Biotech Co., Ltd.

Telephone0411-62910999 13889544652

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