9001-78-9
| Name | EC 3.1.3.1 |
| CAS | 9001-78-9 |
| EINECS(EC#) | 232-631-4 |
| Molecular Formula | NULL |
| MDL Number | MFCD00131849 |
| Molecular Weight | 496.561 |
| MOL File | 9001-78-9.mol |
Synonyms
AP
CAP
AKP
MCAP
IRAP
CIAP
HUNK1
HUNKI
IL1F3
IL-1RN
Ostase
IL-1ra3
ICIL-1RA
CIP/CIAP
alkaline
Detonator
EC 3.1.3.1
EC: 3.1.3.1
IUB: 3.1.3.1
IL-1RA murine
IL1 inhibitor
Einecs 232-631-4
PHOSPHTASE ALKALINE
PHOSPHATASE, ALKALINE
bromodomain containing 4
Alkaline phosphohydrolase
Alkaline phenyl phosphatase
BRD4 (49-170) GST tag human
BRD4 (49-170) His tag human
SHRIMP ALKALINE PHOSPHATASE
ALKALINE PHOSPHATASE, SHRIMP
ALKALINE PHOSPHATASE, BOVINE
PHOSPHATASE, ALKALINE BOVINE
ALKALINE PHOSPHATASE REAGENT
BRD4 (342-460) GST tag human
BRD4 (342-460) His tag human
Phosphatase, Alkaline shrimp
Alkaline phosphomonoesterase
PHOSPHATASE, ALKALINE TYPE IV
PHOSPHATASE, ALKALINE, E COLI
phosphatasealkalinefrome.coli
PHOSPHATASE, ALKALINE TYPE III
PHOSPHATASE, ALKALINE TYPE I-S
ALKALINE PHOSPHATASE YELLOW LI
IL-1 RECEPTOR ANTAGONIST human
PHOSPHATASE, ALKALINE TYPE VIII
PHOSPHATASE, ALKALINE TYPE XVII
PHOSPHATASE, ALKALINE TYPE XXIV
PHOSPHATASE, ALKALINE TYPE XXXII
PHOSPHATASE, ALKALINE TYPE VII-L
PHOSPHATASE, ALKALINE TYPE VII-S
PHOSPHATASE, ALKALINE TYPE VII-T
PHOSPHATASE, ALKALINE TYPE III-L
PHOSPHATASE, ALKALINE TYPE III-S
PHOSPHATASE, ALKALINE TYPE XXXIII
PHOSPHATASE, ALKALINE TYPE VII-NT
PHOSPHATASE, ALKALINE FROM SHRIMP
PHOSPHATASE, ALKALINE TYPE VII-NA
Phosphatase,AlkalineExCalfIntestine
CALF INTESTINE ALKALINE PHOSPHATASE
PHOSPHATASE, ALKALINE CALF INTESTINE
ALKALINE PHOSPHATASE CALF INTESTINAL
CALF INTESTINAL ALKALINE PHOSPHATASE
PHOSPHATASE, ALKALINE, HUMAN PLACENTA
Alkaline Phosphatase from E. coli
PHOSPHATASE, ALKALINE BACTERIAL (BAP)
ANTI-LNPEP antibody produced in mouse
BRD4, BD1, BD2 (49-460) His tag human
BRD4, BD1, BD2 (49-460) GST tag human
phosphatase, alkaline from bovine liver
PHOSPHATASE, ALKALINE TYPE III*BACTERIAL
ALKALINE PHOSPHATASE (CATALYTIC CONCENTR
phosphatase, alkaline from human placenta
Phosphatase, Alkaline from bovine milk
PHOSPHATASE, ALKALINE FROM PORCINE*KIDNE Y
ORTHOPHOSPHORIC-MONOESTER PHOSPHOHYDROLASE
Phosphatase alkaline calf intestinal mucosa
phosphatase, alkaline from rabbit intestine
phosphatase, alkaline from escherichia coli
Phosphatase, Alkaline from Chicken Intestine
phosphatase alk. from calf intestinal mucosa
Phosphatase alkaline bovine intestinal mucosa
BASIC PHOSPHATASE, WHITE LYOPHILIZATED POWDER
ALKALINE PHOSPHATASE MOLECULAR BIOLOGYRE AGENT
Alkaline phosphatase (catalytic concentration)
Phosphatase, Alkaline from canine intestine
PHOSPHATASE, ALKALINE FROM CALF INTESTINE (CIP)
Phosphatase, Alkaline from trout intestine
CIAP, Orthophosphoric-monoester phosphohydrolase
alkaline phosphatase from calf intestinal mucosa
phosphatase alkaline from calf intestinal mucosa
PHOSPHATASE, ALKALINE FROM BOVINE CALF*I NTESTINE
phosphatase alkaline type xvii*from human placent
PHOSPHATASE ALKALINE TYPE XV FROM BOVINE PLACENTA
PHOSPHATASE, ALKALINE, BOVINE*RECOMBINANT PRODUCED
ALKALINE PHOSPHATASE SOLUTION, FROM CALF INTESTINE
PHOSPHATASE ALKALINE TYPE XXIV*FROM HUMA N PLACENTA
PHOSPHATASE ALKALINE TYPE XXXII FROM*BOV INE KIDNEY
5-Allyl-5-(2-hydroxypropyl)-1-phenylbarbituric acid
PHOSPHATASE ALKALINE TYPE XVII*FROM HUMA N PLACENTA
PHOSPHATASE ALKALINE TYPE XII FROM*SHEEP INTESTINE
PHOSPHATASE, ALKALINE TYPE I-S FROM*BOVI NE INTESTIN
PHOSPHATASE, ALKALINE TYPE XXX FROM*CALF INTESTINAL
PHOSPHATASE, ALKALINE TYPE XVIII FROM*PO RCINE INTES
PHOSPHATASE, ALKALINE TYPE XXXIII FROM*B OVINE LIVER
PHOSPHATASE, ALKALINE TYPE III-L*BACTERI AL (ESCHERI
PHOSPHATASE ALKALINE TYPE VII-T FROM*BOV INE INTESTI
PHOSPHATASE ALKALINE TYPE XVI*FROM PORCI NE PLACENTA
PHOSPHATASE ALKALINE TYPE XXIII*FROM TRO UT INTESTIN
PHOSPHATASE ALKALINE TYPE VII-L FROM*BOV INE INTESTI
phosphomonesterase, alkaline, calf intestinal mucosa
Phosphatase, Alkaline from guinea pig intestine
PHOSPHATASE ALKALINE TYPE VII-S FROM*BOV INE INTESTI
PHOSPHATASE ALKALINE TYPE VIII*FROM RABB IT INTESTIN
PHOSPHATASE, ALKALINE FROM BOVINE*INTEST INAL MUCOSA
phosphatase, alkaline from porcine intestinal mucosa
Phosphatase, Alkaline froM Escherichia coli(Purified)
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM]
PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, ~2500 U/MG
PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, ~4200 U/MG
ALKALINE PHOSPHATASE, RECOMBINANT, MOLECULAR BIOLOGY GRADE
PHOSPHATASE ALK. FROM CALF INTESTINAL MU COSA, PWD.~1 U/MG
PHOSPHATASE ALKALINE F. BOV. INTESTINAL MUCOSA, ~2000 U/MG
PHOSPHATASE ALK. FROM CALF INTEST. MUC., STAB., ~1000 U/MG*
AlkalinePhosphatase,(AmmoniumSulfateSuspensionCalfIntestine)
ORTHOPHOSPHORIC-MONOESTER PHOSPHOHYDROLASE (ALKALINE OPTIMUM)
Alkaline Phosphatase from calf intestine ca. 7000 U/mg protein
Alkaline Phosphatase from calf intestine ca. 5 400 U/mg protein
ALKALINE PHOSPHATASE (AP), ORIGIN BOVINE INTESTINE, RECOMBINANT
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE IV
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE I-S
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VIII
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XVII
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXIV
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXXII
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-L
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-S
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE VII-T
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-L
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE III-S
ORTHOPHOSPHORIC-MONOESTER PHOSPHOHYDROLASE (ALKALINE OPTIMUM) BOVINE
PHOSPHORIC MONOESTER PHOSPHOHYDROLASE [ALKALINE OPTIMUM] TYPE XXXIII
5-Allyl-5-(2-hydroxypropyl)-1-phenyl-2,4,6(1H,3H,5H)-pyrimidinetrione
Alkaline Phosphatase from calf intestine ca. 3 000 U/mg protein (ca. 60 U/
Phosphatase alkaline, immobilized on Agarose from calf intestinal mucosa
Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Phosphatase, Alkaline from bovine intestinal mucosa,Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Chemical Properties
| Appearance | solid |
| density | 1.12 g/mL at 20 °C |
| storage temp. | 2-8°C |
| form | suspension |
| color | white |
| Specific Gravity | 1 |
| Stability: | Stability Incompatible with strong oxidizing agents |
| Water Solubility | It is soluble in water. |
| EPA Substance Registry System | Alkaline phosphatase (9001-78-9) |
Safety Data
| Hazard Codes | B,Xi |
| Risk Statements | |
| Safety Statements | |
| RIDADR | UN 0029 1.1B |
| RIDADR | UN 0030 1.1B |
| WGK Germany | 3 |
| F | 3-10 |
| TSCA | Yes |
| HazardClass | 1.1B |
| HS Code | 35079090 |
| Toxicity |
An enzyme with an
alkaline pH optimum that hydrolyzes phosphate monoesters.
Elevation of its activity in the serum usually indicates obstructive
jaundice, Paget’s disease (osteitis deformans), or bone
carcinoma.
|
Hazard Information
Chemical Properties
solid
Usage
Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.
Uses
Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.
Uses
Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.
Uses
Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.
General Description
Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.
Biochem/physiol Actions
Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).
Purification Methods
The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]
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