ChemicalBook CAS DataBase List 9001-78-9

9001-78-9

Name	EC 3.1.3.1
CAS	9001-78-9
EINECS(EC#)	232-631-4
Molecular Formula	NULL
MDL Number	MFCD00131849
Molecular Weight	496.561
MOL File	9001-78-9.mol

Chemical Properties

Appearance	solid
density	1.12 g/mL at 20 °C
storage temp.	2-8°C
form	suspension
color	white
Specific Gravity	1
PH	~7.6
Stability:	Stability Incompatible with strong oxidizing agents
biological source	mouse
Water Solubility	It is soluble in water.
Specific Activity	30-90units/mg protein (modified Warburg-Christian, in glycine buffer)
Cosmetics Ingredients Functions	SKIN CONDITIONING
InChIKey	ITZMJCSORYKOSI-XUAWLZQMNA-N
SMILES	C(O)(=O)[C@@H](NC([C@@H]1CCCN1C(CNC([C@@H]1CCCN1C(=O)[C@@H](N)C)=O)=O)=O)CCC/N=C(\N)/N \|&1:3,6,15,22,r\|
EPA Substance Registry System	Alkaline phosphatase (9001-78-9)
Absorption	7.6 at 278nm (10 mg AP/ml)

Safety Data

Hazard Codes	B,Xi
Risk Statements	36/37/38 less more
Safety Statements	26-36-24/25-22-23 less more
RIDADR	UN 0029 1.1B
RIDADR	UN 0030 1.1B
WGK Germany	3
F	3-10
TSCA	Yes
HazardClass	1.1B
HS Code	35079090
Storage Class	11 - Combustible Solids
Hazard Classifications	Resp. Sens. 1
Toxicity	An enzyme with an alkaline pH optimum that hydrolyzes phosphate monoesters. Elevation of its activity in the serum usually indicates obstructive jaundice, Paget’s disease (osteitis deformans), or bone carcinoma. less more

Raw materials And Preparation Products

Hazard Information

Chemical Properties

solid

Usage

Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.

Uses

Alkaline Phosphatase is commonly used to remove the 5′-terminal phosphate from nucleic acids.

Uses

Alkaline Phosphatase, calf intestine, EIA GradeDephosphorylation of cloning vector DNA to prevent recircularization during ligation, dephosphorylation of DNA prior to end-labeling using T4 Polynucleotide Kinase, treatment of dNTPs in PCR reactions prior to sequencing or SNP analysis, dephosphorylation of DNA and RNA. It is also used to remove the 5?-terminal phosphate from nucleic acids during molecular cloning reactions.

Uses

Hydrolyzes 5′-terminal monophosphate (dephosphorylation) from DNA and RNA. Prevents fragments from self annealing. 5′-nucleic acid targeting for probes.

General Description

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Biochem/physiol Actions

Alkaline phosphatase is a model enzyme for understanding phosphomonoesterase. It is used in various biochemical methods and enzyme linked immunosorbent assay (ELISA).

Purification Methods

The E.coli supernatant in sucrose (20%, 33mM) in Tris-HCl pH 8.0 is purified through a DEAE-cellulose column and recrystallised. To the column eluates in 0.125M NaCl is added MgCl2 (to 0.01M) and brought to 50% saturation in (NH4)2SO4 by adding the solid (0.20g/mL). The mixture is centrifuged to remove bubbles and is adjusted to pH 8.0 (with 2N NaOH). Saturated (NH4)2SO4 at pH 8.0 is added dropwise until the solution becomes faintly turbid (~61% saturation). It is set aside at ~25o for 1hour (turbidity will increase). The mixture is placed in an ice bath for several minutes when turbidity disappears and a clear solution is obtained. It is then placed in a large ice bath at 0o (~5L) and allowed to warm slowly to room temperature in a dark room whereby crystals are formed appearing as a silky sheen. The crystals are collected by centrifugation at 25o if necessary. The crystalline solutions are stable at ~25o for many months. They can be stored at 0o, but are unstable when frozen. Cysteine at 10-3M and thioglycolic acid at 10-4M are inhibitory. This is reversed on addition of Zn2+ ions. Many organic phosphates are good substrates for this phosphatase. [Molamy & Horecker Methods Enzymol 9 639 1966, Torriani et al. Methods Enzymol 12b 212 1968, Engstrom Biochim Biophys Acta 92 71 1964.] Alkaline phosphatase from rat osteosarcoma has been purified by Me2CO precipitation and chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxylapatite. [Nair et al. Arch Biochem Biophys 254 18 1987.] Phosphoproteins (various). These are purified by adsorbing onto an iminodiacetic acid substituted agarose column to which are bound ferric ions. This chelate complex acts as a selective immobilised metal affinity adsorbent for phosphoproteins. [Muszyfiska et al. Biochemistry 25 6850 1986.]

Supplier

Hefei Bomei Biotechnology Co., Ltd.

Telephone13739298932 13856598764

Websitehttp://www.bomeibio.com

Hzymes Biotechnology Co. LTD

Telephone400-8085320 16621234168

Websitehttps://www.hzymes.com/

Shanghai Baoman Biotechnology Co., Ltd.

Telephone021-62130998

Websitehttp://www.baomanbio.com

Vazyme Biotech Co.,Ltd

Telephone 18260633103

Website

Hubei wei shi reagent group ltd., company

Telephone13125137732 18686406941

Websitehttps://www.4008081911.com

Nanjing Dulai Biotechnology Co., Ltd.

Telephone025-84699383-8003;025-846993838003-8003 18013301590

Websitehttp://www.njduly.com

Beijing Jin Ming Biotechnology Co., Ltd.

Telephone010-60605840

Websitehttp://www.jm-bio.com/

J & K SCIENTIFIC LTD.

Telephone18210857532 18210857532

Websitehttps://www.jkchemical.com

Meryer (Shanghai) Chemical Technology Co., Ltd.

Telephone4006356688 18621169109

Websitehttp://www.x-labseek.com/

Beijing HwrkChemical Technology Co., Ltd

Telephone89508211 18501085097

Websitehttp://www.hwrkchemical.com

Energy Chemical

Telephone 400-0056266

Websitehttp://www.energy-chemical.com

Adamas Reagent, Ltd.

Telephone4006009262 13023226327

Websitehttp://www.tansoole.com

Shanghai Longsheng chemical Co.,Ltd.

Telephone58099637 13585536065

Websitehttp://www.shlshg.com

Shandong Xiya Chemical Co., Ltd

Telephone13355009207 13355009207

Websitehttp://www.xiyashiji.com

Chengdu Chuanke Fine Chemicals C.,Ltd.

Telephone+86 (28) 68626087

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList15180/0_EN.htm

Tianjin heowns Biochemical Technology Co., Ltd.

Telephone400 638 7771

Websitehttp://www.heowns.com

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Telephone0731-85526065 13308475853

Websitehttp://www.hnhbsj.com/

Dalian Meilun Biotech Co., Ltd.

Telephone0411-62910999 13889544652

Websitehttp://www.meilunbio.com/

1of4

Related Products

Send Inquriy