ChemicalBook CAS DataBase List 9001-50-7

9001-50-7

Name	GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
CAS	9001-50-7
EINECS(EC#)	232-609-4
Molecular Formula	C3H7O6P
MDL Number	MFCD00131203
Molecular Weight	170.058
MOL File	9001-50-7.mol

Chemical Properties

storage temp.	2-8°C
form	suspension
color	white
EPA Substance Registry System	Dehydrogenase, glyceraldehyde phosphate (9001-50-7)

Safety Data

Hazard Codes	B,Xi
Risk Statements	36/37/38 less more
Safety Statements	26-36 less more
WGK Germany	3

Hazard Information

Uses

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:

for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
in anti-aggregation assays
in purified GAPDH studies

General Description

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.

Biochem/physiol Actions

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>.

Purification Methods

Purify the dehydrogenase from rabbit muscle by extraction with 0.03N KOH and precipitate it with (NH4)2SO4 (0.52 of saturation). The clear supernatant is adjusted to pH 7.5, and NH3 is added dropwise to pH 8.2-8.4. Crystals appear sometimes even without seeding. The crystals are dissolved in H2O, filtered to remove suspended material and 2 volumes of saturated (NH4)2SO4 at pH 8.2-8.4 is added. After 1hour the crystals appear. Recrystallise it in the same way. [Cori et al. J Biol Chem 173 605 1948, Furfine & Velick J Biol Chem 240 844 1965, The Enzymes 7 243 1963, Lui & Huskey Biochemistry 31 6998 1992.] The Km values are: NADH (3.3WM) and 1,3-diphosphoglycerate (8x10-7M) in pH 7.4 imidazole buffer at 26o, NAD (13WM), glyceraldehyde-3-P (90WM), Pi (2.9x10-4M), and arsenate (69WM) in 8.6 M NaHCO3 buffer at 26oC. [Orsi & Cleland Biochemistry 11 102 1972.]

Supplier

Shanghai Yingxin laboratory equipment Co., Ltd

Telephone021-021-59178156 15300768757

Websitehttp://www.yingxinbio.com/

Shanghai Guchen Biotechnology Co., LTD

Telephone021-34675735 19147740836

Websitehttp://www.shgcsw-edu.com

Adamas Reagent, Ltd.

Telephone400-6009262 16621234537

Websitehttp://www.tansoole.com

Sichuan Kulinan Technology Co., Ltd

Telephone400-1166-196 18981987031

Websitehttp://www.hx-r.com/

ShangHai YuanYe Biotechnology Co., Ltd.

Telephone021-61312847 13636370518

Websitehttp://www.shyuanye.com

Shanghai Ruji Biology Technology Co., Ltd.

Telephone+86-21-65211385-8001 36031160

Websitehttp://www.chinaruji.com/

TargetMol Chemicals Inc.

Telephone021-021-33632979 15002134094

Websitehttps://www.targetmol.cn/

Shanghai BeiZhuo Biotech Co., Ltd.

Telephone021-61119791,13386096464

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList16081/0.htm

Sigma-Aldrich

Telephone021-61415566 800-8193336

Websitehttps://www.sigmaaldrich.cn

Beijing NuoqiYa Biotechnology Co., Ltd.

Telephone4000-819-385 010-62329685 13718666987

Websitehttp://www.bjoka-vip.com

Shandong Xiya Chemical Co., Ltd.

Telephone4009903999 13395398332

Websitehttp://www.xiyashiji.com

Raw material medicin reagent co.,Ltd

Telephone

Websitehttp://www.njromanme.com

Beijing Solarbio Science & Tecnology Co., Ltd.

Telephone010-50973130 4009686088

Websitehttp://www.solarbio.com

Shanghai Civic Chemical Technology Co., Ltd.

Telephone021-34053660

Websitehttp://www.civi-chem.com

Amatek Scientific Co. Ltd.

Telephone0512-56316828

Websitehttp://www.amateksci.com

Guangzhou LES biological Technology Co.,Ltd.

Telephone 13672434928

Websitehttp://www.les64.com

Hubei widely chemical technology Co., Ltd.

Telephone027-83778895 18602735115

Websitehttp://www.hbwidely.cn

Shanghai Jizhi Biochemical Technology Co. Ltd.

Telephone4009004166/18616739031 18616739031

Websitehttp://www.acmec-e.com/

1of4

Related Products

Send Inquriy