ChemicalBook CAS DataBase List 9001-32-5

9001-32-5

Name	HUMAN FIBRINOGEN
CAS	9001-32-5
EINECS(EC#)	232-598-6
Molecular Formula	NULL
MDL Number	MFCD00131060
Molecular Weight	182.172
MOL File	9001-32-5.mol

Chemical Properties

Definition	A sterile fraction of normal human blood plasma, dried from the frozen state. In solution, it has the property of being converted into insoluble fibrin when thrombin is added. It is an essential factor in the blood-clotting mechanism.
Appearance	White or grayish amorphous substance.
storage temp.	−20°C
solubility	0.9% NaCl: soluble
form	powder
color	white
biological source	r at plasma
Water Solubility	water: 25mg/mL
InChI	InChI=1/C6H14O6/c7-1-3(9)5(11)6(12)4(10)2-8/h3-12H,1-2H2/t3-,4-,5+,6+/s3
InChIKey	FBPFZTCFMRRESA-ZCLOERBHNA-N
SMILES	C(O)[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)CO \|&1:2,4,6,8,r\|
EPA Substance Registry System	Fibrinogens (9001-32-5)

Safety Data

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H332-H302-H312
Precautionary statements	P261-P271-P304+P340-P312-P264-P270-P301+P312-P330-P501-P280-P302+P352-P312-P322-P363-P501
Hazard Codes	B
WGK Germany	3
F	10-21
TSCA	Yes
Storage Class	13 - Non Combustible Solids

Hazard Information

Chemical Properties

White or grayish amorphous substance.

Uses

Diagnostic aid (vascular patency); radioactive agent.

General Description

Fibrinogen, or Factor I, is a blood protein that is involved in clotting and is converted to fibrin by thrombin. Fibrinogen has an approximate molecular weight of 340 kDa

Biochem/physiol Actions

Fibrinogen is an acute phase protein that is part of the coagulation cascade of proteins. The end result of the cascade is the production of thrombin that converts fibrinogen to fibrin. Thrombin rapidly proteolyses fibrinogen, releasing fibrinopeptide A. The loss of this small peptide is not sufficient to make the resulting fibrin molecule insoluble, but it tends to form complexes with adjacent fibrin and fibrinogen molecules. Thrombin then cleaves a second peptide, fibrinopeptide B, from fibrin and the fibrin monomers formed then polymerize spontaneously to form an insoluble gel. The polymerized fibrin is held together by noncovalent and electrostatic forces and stabilized by the transamidating enzyme, factor XIIIa, that is produced by the action of thrombin on factor XIII. The insoluble fibrin aggregates (clots) and aggregated platelets then block the damaged blood vessel and prevent further bleeding. The amount of fibrinogen in the plasma can serve as a nonspecific indicator of whether or not an inflammatory process is present in the body. Fibrinogen from any mammalian source will be cleaved by thrombin from any mammalian source.

Synthesis

China has abundant pig blood resources, but due to the production process conditions are not mature enough, resulting in the utilization of pig blood resources is less than 1%. 2006 for the first time to use freeze-thawing method to extract pig blood fibr

Purification Methods

A likely impurity is plasminogen. It is purified by glycine precipitation [Mosesson & Sherry Biochemistry 5 2829 1966] to obtain fractions 1-2, then further purified [Blomb.ck & Blomb.ck Arkiv Kemi 10 415 1956] and contaminating plasminogen is removed by passage through a lysine-Sepharose column. Such preparations are at least 95% clottable as determined by Mosesson and Sherry's method (above ref.) in which the OD280 is measured before and after clotting with 5 Units/mL of thrombin (> 3000U/mg). All fibrinogen preparations are treated with calf intestinal alkaline phosphatase to convert any fibrinogen peptide-AP to fibrinogen peptide-A by removing serine-bound phosphate. Solutions are then lyophilised and stored at -20o. [Higgins & Shafer J Biol Chem 256 12013 1981.] It is sparingly soluble in H2O. Aqueous solutions are viscous with isoelectric point at pH 5.5. It is readily denatured by heating above 56o or by chemical agents, e.g. salicylaldehyde, naphthoquinone sulfonates, ninhydrin or alloxan. [Edsall et al. J Am Chem Soc 69 2731 1947, Purification: Cama et al. Naturwissenschaften 48 574 1961, Lorand & Middlebrook Science 118 515 1953, cf. Fuller in Methods Enzymol 163 474 1988.] For plasminogen-deficient fibrinogen from blood plasma, the anticoagulated blood is centrifuged and the plasma is frozen and washed with saline solution. It is treated with charcoal, freeze-thawed and dialysed versus Tris/NaCl buffer. [Maxwell & Nikel Biochemical Preparations 12 16 1968.]

Supplier

Shanghai Baoman Biotechnology Co., Ltd.

Telephone021-62130998

Websitehttp://www.baomanbio.com

JiangSu Star Science Co.,Ltd

Telephone400-605-7200 15335295587

Websitehttps://www.b2star.com/

Nanjing Dulai Biotechnology Co., Ltd.

Telephone025-84699383-8003;025-846993838003-8003 18013301593;18013301590

Websitehttp://www.njduly.com

J & K SCIENTIFIC LTD.

Telephone18210857532; 18210857532

Websitehttps://www.jkchemical.com

future industrial shanghai co., ltd

Telephone400-0066400 13621662912

Websitehttp://www.jonln.com

Energy Chemical

Telephone021-021-58432009 400-005-6266

Websitehttp://www.energy-chemical.com

Adamas Reagent, Ltd.

Telephone400-6009262 15618770481

Websitehttp://www.tansoole.com

Shandong Xiya Chemical Co., Ltd

Telephone13355009207 13355009207

Websitehttp://www.xiyashiji.com

Sichuan Kulinan Technology Co., Ltd

Telephone400-1166-196 18981987031

Websitehttp://www.hx-r.com/

Sinopharm Chemical Reagent Co,Ltd.

Telephone86-21-63210123

Websitehttp://www.reagent.com.cn

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Telephone0731-85526065 13308475853

Websitehttp://www.hnhbsj.com/

Maya High Purity Chemicals

Telephone+86 (573) 82222445 (0)18006601000 452520369

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList15221/0_EN.htm

Wuhan Fortuna Chemical Co., Ltd

Telephone027-59207852 13308628970

Websitehttp://www.fortunachem.cn

Dalian Meilun Biotech Co., Ltd.

Telephone0411-62910999 13889544652

Websitehttp://www.meilunbio.com/

ShangHai YuanYe Biotechnology Co., Ltd.

Telephone 13636370518

Websitehttp://www.shyuanye.com

Beijing HuaMeiHuLiBiological Chemical

Telephone010-56205725

Websitehttp://www.huabeibiochem.com/

Shanghai Aladdin Bio-Chem Technology Co.,LTD

Telephone400-6206333 13167063860

Websitehttp://www.aladdin-e.com/

Shanghai Ruji Biology Technology Co., Ltd.

Telephone+86-21-65211385-8001 36031160

Websitehttp://www.chinaruji.com/

1of4

Related Products

Send Inquriy