ChemicalBook CAS DataBase List 9001-12-1

9001-12-1

Name	Collagenase
CAS	9001-12-1
EINECS(EC#)	232-582-9
Molecular Formula	NULL
MDL Number	MFCD03456440
Molecular Weight	849.449
MOL File	9001-12-1.mol

Chemical Properties

Definition	A rare proteinase that catalyzes the hydrolysis of collagen. Collagenase activity has been demonstrated in several venoms of crotalid and viperid snakes and it occurs in certain species of clostridium.
storage temp.	2-8°C
solubility	Soluble in aqueous buffers.
form	powder
color	light brown
PH	7.0
biological source	Clostridium histolyticum
Water Solubility	water: soluble
Merck	2481
Specific Activity	0.5-5.0FALGPA units/mg solid
InChIKey	YRQNKMKHABXEJZ-UVQQGXFZSA-N
CAS DataBase Reference	9001-12-1
EPA Substance Registry System	Collagenase(9001-12-1)

Safety Data

Symbol(GHS)	GHS07,GHS08
Signal word	Danger
Hazard statements	H315-H319-H334-H335
Precautionary statements	P261-P264-P280-P284-P304+P340+P312-P342+P311
Hazard Codes	Xn
Risk Statements	36/37/38-42 less more
Safety Statements	22-24-26-36/37 less more
WGK Germany	1
F	10-21
TSCA	TSCA listed
HS Code	3507907000

Hazard Information

Uses

This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

General Description

Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.

Biochem/physiol Actions

Effective release of cells from tissue requires the action of collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca2+) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA)4; β-mercaptoethanol; glutathione, reduced; thioglycolic acid, sodium; and 2,2′-dipyridyl; 8-hydroxyquinoline are known to inhibit the enzyme activity.

Purification Methods

Collagenase is purified by using N-ethylmaleimide to activate the enzyme, and wheat germ agglutinin-agarose affinity chromatography [Callaway et al. Biochemistry 25 4757 1986].

Material Safety Data Sheet(MSDS)

Questions And Answer

Description
Collagenases are a category of enzymes that can digest native collagen, the major fibrous component of animal extracellular connective tissue. Collagenases can be produced by some bacterias such as Clostridium and can also be made by the animal body as part of its normal immune response. They normally target the connective tissue in muscle cells and other body organs. They can break peptide bonds within collagen and help create collagen by cleaving pro-collagen, collagen's precursor, once it is secreted by the cell. Bacterial collagenases exhibit broader substrate specificity than animal collagenases and unlike animal collagenases that split collagen in its native triple-helical conformation, bacterial collagenase can degrade both water-insoluble native collagens and water-soluble denatured ones. In addition, bacterial collagenase can attack almost all collagen types, and is able to make multiple cleavages within triple helical regions. Collagenases have a molecular weight ranging from 68 kilodaltons (kDa) to 130 Kda due to differing sizes based on the class to which the isolate belongs. The optimal pH for stability ranges from approximately 6.3 to 7.5 with a theoretical isoelectric point of 5.62. Numerous compounds and molecules have the capability of acting as collagenases activator or inhibitor. For example, Ca2+ and Zn2+ ions are two known activators. On the other hand, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), cysteine, histidine, dithiothreitol (DTT), 2-mercaptoethanol, o-phenanthroline, Hg2+, Pb2+, Cd2+, Cu2+, and Zn2+ are all known inhibitors of collagenase activity. ;
References
1.https://en.wikipedia.org/wiki/Collagenase
2.http://www.wisegeek.com/what-is-collagenase.htm
3.http://www.rockland-inc.com/collagenase.aspx
4.http://www.worthington-biochem.com/cls/default.html ;

Supplier

Beijing Ackerman Technology Co., Ltd.

Telephone 18001219251

Websitehttps://www.ackerman.cn/

Nanjing Dulai Biotechnology Co., Ltd.

Telephone025-84699383-8003;025-846993838003-8003 18013301590

Websitehttp://www.njduly.com

Jiangsu Duoyang Bioengineering Technology Co., Ltd

Telephone 15161148838

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList739813/0_EN.htm

Hunan Wokai Biotechnology Co., Ltd.

Telephone0744-2221508 13126795102

Websitehttp://www.oka-vip.com

Hubei Weideli Chemical Reagent Co., Ltd.

Telephone027-83984790 17720532645

Websitehttps://www.chemicalbook.com/

Hunan Zhangjiajie Hengkang Biological Pharmaceutical Co., LTD

Telephone0731-85561023 18973109536

Websitehttp://www.hengkangapi.com

Hubei Weideli Chemical Reagent Co.，Ltd.

Telephone17771473825 13385289472

Websitehttps://www.chemicalbook.com/supplier/23855892/1.htm

Absin Bioscience Inc.

Telephone 13761418683

Websitehttps://www.absin.cn/

J & K SCIENTIFIC LTD.

Telephone18210857532 18210857532

Websitehttps://www.jkchemical.com

3B Pharmachem (Wuhan) International Co.,Ltd.

Telephone 18930552037

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList13285/0_EN.htm

Adamas Reagent, Ltd.

Telephone400-6009262 15618770481

Websitehttp://www.tansoole.com

Shandong Xiya Chemical Co., Ltd

Telephone13355009207 13355009207

Websitehttp://www.xiyashiji.com

Sichuan Kulinan Technology Co., Ltd

Telephone400-1166-196 18981987031

Websitehttp://www.hx-r.com/

Tianjin heowns Biochemical Technology Co., Ltd.

Telephone400 638 7771

Websitehttp://www.heowns.com

Hunan Hui Bai Shi Biotechnology Co., Ltd.

Telephone0731-85526065 13308475853

Websitehttp://www.hnhbsj.com/

Maya High Purity Chemicals

Telephone+86 (573) 82222445 (0)18006601000 452520369

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList15221/0_EN.htm

Spectrum Chemical Manufacturing Corp.

Telephone021-021-021-67601398-809-809-809 15221380277

Websitehttps://www.spectrumchemical.com/OA_HTML/index.jsp?minisite=10020&respid=22372&language=US

Dalian Meilun Biotech Co., Ltd.

Telephone0411-62910999 13889544652

Websitehttp://www.meilunbio.com/

1of4

Related Products

Send Inquriy