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Rhod-5N is a low affinity fluorescent calcium probe (K_d = 320 μM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes will be insensitive to luminal fluctuations. Rhod-5N has excitation/emission maxima of approximately 551 and 576 nm, respectively

Rhod-5N is a calcium-binding fluorescent dye composed of a BAPTA chelating group and a rhodamine fluorophore. Rhod-5N is often added to MOPS buffer to complex and indicate cation content. The selectivity of Rhod-5N for Cd2+ is higher than other interfering cations ((Na+, K+, Mg2+, Ca2+, Zn2+) except Pb2+), and the detection limit is 3.1 μg/L[1][2].

Store at -20°C

[1] Soibinet M, Souchon V, Leray I, et al. Rhod-5N as a fluorescent molecular sensor of cadmium (II) ion[J]. Journal of fluorescence, 2008, 18: 1077-1082.
[2] McNamara C J, Perry Iv T D, Bearce K, et al. Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N[J]. Journal of microbiological methods, 2005, 61(2): 245-250.