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Fluo-3 (potassium salt) is a fluorescent calcium indicator commonly used in flow cytometry and cell-based experiments to detect changes in intracellular calcium levels. Its absorption maximum at 506 nm makes it compatible with excitation at 488 nm by argon-ion laser sources. Fluo-3 provides intense fluorescence upon binding calcium, detected at a maximum emission at 526 nm which can be monitored by FL1 (green, 525 nm band pass) sensors in flow cytometry.

Fluo-3 pentapotassium is a fluorescent indicator for intracellular Ca2+ that is used in flow cytometry and cell-based experiments. Fluo-3 pentapotassium produces fluorescence with an emission maximum at 525 nm upon binding to calcium.