82005-44-5

Oleic acid-¹³C is intended for use as an internal standard for the quantification of oleic acid (Item Nos. 90260 | 24659) by GC- or LC-MS. Oleic acid is a monounsaturated fatty acid and a major component of membrane phospholipids that has been found in human plasma, cell membranes, and adipose tissue. It contributes approximately 17% of the total fatty acids esterified to phosphatidylcholine, the major phospholipid class in porcine platelets. Oleic acid inhibits collagen-stimulated platelet aggregation by approximately 90% when used at a concentration of 10 μg/ml. It also inhibits fMLF-induced neutrophil aggregation and degranulation by 55 and 68%, respectively, when used at a concentration of 5 μM, similar to arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607). Oleic acid (60 μM) induces release of intracellular calcium in human platelets. In vivo, oleic acid increases TNF-α, IL-8, IL-6, and IL-1β production, neutrophil accumulation, and apoptotic and necrotic cell death in mouse lung and has been used to induce lung injury in a mouse model of acute respiratory distress syndrome (ARDS).

Labelled Oleic acid. Obtained by the hydrolysis of various animal and vegetable fats and oils.

[1] KLAUS W.J WAHLE  Lesley I L P. Effects of isomeric cis and trans eighteen carbon monounsaturated fatty acids on porcine platelet function[J]. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1996, 1301 1: Pages 141-149. DOI: 10.1016/0005-2760(96)00025-2
[2] C. GON?ALVES-DE ALBUQUERQUE. Acute Respiratory Distress Syndrome: Role of Oleic Acid-Triggered Lung Injury and Inflammation[J]. Mediators of Inflammation, 2015, 2015 1. DOI: 10.1155/2015/260465
[3] P.H. NACCACHE. Modulation of Rabbit Neutrophil Aggregation and Degranulation by Free Fatty Acids[J]. Journal of Leukocyte Biology, 1984, 36 3: 333-340. DOI: 10.1002/jlb.36.3.333
[4] ATHANASSIA SIAFAKA-KAPADAI  Martin A J  Donald J Hanahan. Oleic acid-induced Ca2+ mobilization in human platelets: is oleic acid an intracellular messenger?[J]. Journal of lipid mediators and cell signalling, 1997, 15 3: Pages 215-232. DOI: 10.1016/s0929-7855(96)00554-8