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3-Azido-7-hydroxycoumarin, is a Fluorescent dye that has shown to quickly and efficiently label proteins in bicarbonate buffered solns. ( Abs/Em = 404/477 nm )

This special UV active chromophore is ideal for imaging purposes after conjugation to biomolecules. It emits fluorescence only after the click reaction when its electron system is expanded. Therefore no background signal is expected for the unreacted compound. λabs 404 nm (after click reaction), λem 477 nm (after click reaction ).

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