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CENTA is a colorimetric cephalosporin substrate for β-lactamases. Upon hydrolysis by β-lacatamases, CENTA turns from light yellow to chrome yellow, which can be quantified by colorimetric detection at 405 nm as a measure of β-lactamase activity.

[1] Jones, et al. In vitro evaluation of CENTA, a new beta-lactamase-susceptible chromogenic cephalosporin reagent. J. Clin. Microbiol. 15(5), 954-958 (1982).