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D-Pro-Phe-Arg-pNA is a colorimetric substrate for plasma kallikrein. Plasma kallikrein preferentially binds to and cleaves the D-Pro-Phe-Arg (PFR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of plasma kallikrein activity.

D-Pro-Phe-Arg-pNA hydrochloride is the substrate for thrombin-like enzyme, with Km of 50.7 μM[1].

[1] Magalh?es HP, et al., Mechanism of action and determination of the best substrate for a thrombin-like enzyme from Lachesis muta muta venom by regression analysis of the kinetic parameters determined with peptidyl p-nitroanilide substrates. Toxicon. 2006 Mar 15;47(4):453-8. DOI:10.1016/j.toxicon.2006.01.001