510758-19-7

FAM (fluorescein) alkyne is a high purity (95%) 5-isomer with significant aqueous solubility. Alkyne enables copper-catalyzed Click chemistry.

5-FAM-Alkyne is a high selective and sensitive fluorescent biosensor for alkaline phosphatase (ALP)[1]. 5-FAM-Alkyne is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

[1] Mengmeng Zhao, et al. A Sensitive Fluorescence Biosensor for Alkaline Phosphatase Activity Based on the Cu(II)-dependent DNAzyme. Anal Chim Acta. 2016 Dec 15;948:98-103. DOI:10.1016/j.aca.2016.10.033

492/517 nm

0.93

74000

0.22

0.17