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MRT10 is an antagonist of the Smoothened (Smo) receptor with an IC₅₀ value of 0.64 μM in a luciferase reporter assay. It also inhibits bodipy-cyclopamine binding to the murine Smo receptor (IC₅₀ = 0.5 μM) when expressed in HEK293 cells.

MRT 10, is a novel, high-affinity antagonist smoothened (Smo) receptor antagonist. It displaces labeled cyclopamine from Smo with an IC50 of 500 nM, and inhibits shh-induced Gli-reporter activity in Shh-light2 cells (IC50 = 0.64 μM).

[1] FABRIZIO MANETTI. Virtual screening-based discovery and mechanistic characterization of the acylthiourea MRT-10 family as smoothened antagonists.[J]. Molecular Pharmacology, 2010, 78 4: 658-665. DOI: 10.1124/mol.110.065102
[2] ANTONIO SOLINAS. Acylthiourea, Acylurea, and Acylguanidine Derivatives with Potent Hedgehog Inhibiting Activity[J]. Journal of Medicinal Chemistry, 2012, 55 4: 1559-1571. DOI: 10.1021/jm2013369