33016-12-5

A novel synthetic inhibitor of microtubule formation which binds to the colchicine site. It prevents the stabilization of microtubules in taxol-treated cells. Blocks the cell cycle in M-phase.

3-(l-anilinoethylidene)-5-benzylpyrrolidine-2,4-dione (TN-16)a tenuazonic acid derivativeis a synthetic antitumor agent which arrests mitosis of tumor cells at metaphase. It is a colchicine site binding agent and a powerful autophagy flux inhibitor th at could be used as a conventional autophagy inhibitor with anti-cancer potential in pre-clinical trials.''Inhibitor of microtubule assembly''TN-16 is a synthetic compound th at blocks the assemblyand promotes the dissociation of microtubules. It can prevent the b-TrCP-mediated degradation of the transcription factor Gli2leading to the up-regulation of BMP-2 and supporting a role for microtubule formation in the regulation of bone formation.