254117-01-6

C12-NBD-sphingomyelin has been used as a substrate to test substrate specificity of neutral ceramidase orthologue encoded by AsahA gene.

Sphingomyelin (SM) is produced from phosphatidylcholine and ceramide on the cell surface and in lumen of the Golgi. SMμs high packing density and affinity to sterols form a rigid barrier to the extracellular environment. SM is also involved in the formation of lipid rafts in cell membraneswhich perform important functions in signal transduction and membrane trafficking.