2360411-64-7

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
? Cell tracking in vivo^[1][2]:
? 1. Seed cells onto 35-mm glass-bottom dishes at a density of 3×10⁴ (varies by cell) in 2 mL of growth media.
? 2. Add Ac4ManNAz (50 μM, final concentration), incubate for 3 days (generate azide groups on the surface of cells).
? 3. Wash cells twice with DPBS (pH 7.4).
? 4. Anesthesia for mice, then inject cells (after Ac4ManNAz treatment) into the left lobe of the liver.
? 5. Inject Cyanine5 DBCO (25 μM, 200 μL) to tail vein (3 days post injection of cells into the liver).
? 6. Measure Cyanine5 DBCO fluorescence (Ex=635 nm, Em=650-700 nm). In vivo near-infrared fluorescence (NIRF) images is obtained with the in vivo imaging system.