DMHBO+ is a cationic chromophore that binds to the Chili aptamer with a Kd of 12 nM. The Chili-DMHBO+ complex is an ideal fluorescence donor for FRET to the rhodamine dye Atto 590, suitable for imaging RNA in cells. Ex/Em=456/592 nm[1].
References
[1] Christian Steinmetzger, et al. A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores. Chemistry. 2019 Feb 6;25(8):1931-1935. DOI:10.1002/chem.201805882
