Uses
DDMS is used as an irreversible inhibitor that has selectivity for the CYP4A2 enzyme which synthesizes 20-HETE in the mammalian kidney. 20-HETE plays an important role in vascular homeostasis.
Biological Activity
ddms is a cyp4a2 enzyme inhibitor.biosynthesis of 20-hete from arachidonic acid by the cytochrome p450 4a (cyp450 4a) isoforms is a key component of vascular homeostasis, especially in renal circulation.
in vitro
to determine whether inhibition of 20-hete contributes to the vasodilatory effects of no, the effects of ddms on the response to snp were examined in rat renal arterioles preconstricted with phenylephrine). results showed that after ddms treatment, snp could increase vascular diameter by only 17% [1].
in vivo
the effects of ddms on the mean arterial pressure and renal blood flow responses to infusion of an no donor and a synthase inhibitor were also examined. it was found that infusion of mahma nonoate at 1, 3, 5, and 10 nmol/min was able to reduce mean arterial pressure by 16, 30, 40, and 48 mm hg and lowered renal vascular resistance by 15 %, 26 %, 30%, and 34% of control. in addition, after ddms treatment at 10 mg/kg, the mean arterial pressure and renal vascular resistance responsed to 1-hexamine, 6-(2-hydroxy-1-methyl-2-nitrohydrazino)n-methyl averaged only 20% of those seen during control [1].
References
[1] alonso-galicia, m. ,drummond, h.a.,reddy, k.k., et al. inhibition of 20-hete production contributes to the vascular responses to nitric oxide. hypertension 29, 320-325 (1997).
