180854-97-1

CM-DiI is an orange-red fluorescent carbocyanine dye containing thiol-reactive chloromethyl (CM) moiety. CM-DiI labels plasma membranes by inserting its two long hydrocarbon (C18 carbon) chains into the lipid bilayer. The dye is weakly fluorescent until incorporated into membranes.

CM-DiI is used for in vivo and in vitro labeling of cells to analyze their proliferation as well as for cell tracking and motility assays. The dye is transferred to daughter cells and does not leak to adjacent cells in a population. CM-DiI staining displays bright fluorescence for at least 72 hours (around three to six cell generations). The dye shows little cytotoxicity inside the cell, with minimal effects on the proliferative ability or biology of the cell.

Unlike other membrane dyes, CM-DiI has increased water solubility and can be fixed using traditional aldehyde fixatives. CM-DiI also persists in cells after permeabilization procedures. These make it feasible to detect labeled cells in combination with other histological techniques such as immunochemistry, optical clearing, etc.

CellTracker CM-DiI (CM-DiI) is a lipophilic fluorochrome that can be used for tracking the migration of lymphocytes. CellTracker CM-DiI is feasible to detect labeled cells in histological sections (excitation 553 nm; emission 570 nm)[1][2][3].

[1] Andrade W, et al. The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes. J Immunol Methods. 1996 Aug 14;194(2):181-9. DOI:10.1016/0022-1759(96)00083-x
[2] Dupuis M, et al. Distribution of adjuvant MF59 and antigen gD2 after intramuscular injection in mice. Vaccine. 1999 Oct 14;18(5-6):434-9. DOI:10.1016/s0264-410x(99)00263-7
[3] Rutten M, et al. Comparison of quantum dots and CM-DiI for labeling porcine autologous bone marrow mononuclear progenitor cells. The Open Stem Cell Journal, 2010, 2(1).

556/571 nm

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