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Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Desthiobiotin-PEG4-alkyne is a biotinylated biochemical reagent, which can be utilized in conjunction with click chemistry for the selective labeling and enrichment of certain tRNAs[1].

[1] Krasheninina OA, et al., Amine-to-Azide Conversion on Native RNA via Metal-Free Diazotransfer Opens New Avenues for RNA Manipulations. Angew Chem Int Ed Engl. 2021 Mar 22;60(13):6970-6974. DOI:10.1002/anie.202015034