ChemicalBook CAS DataBase List 148504-34-1

148504-34-1

Name	CALCEIN-AM
CAS	148504-34-1
EINECS(EC#)	200-664-3
Molecular Formula	C46H46N2O23
MDL Number	MFCD00282766
Molecular Weight	994.86
MOL File	148504-34-1.mol

Synonyms

CAL-AM NSC 689290 CALCEIN-AM AM /Calcein-AM CALCEIN, AM;CAL-AM Calcein AM solution Cellstain- Calcein-AM Calcein AcetoxyMethyl Ester -Cellstain-Calcein-AmSolution Calcein, AM *CAS 148504-34-1* CALCEIN AM, 4MM IN ANHYDROUS DMSO CALCEIN-AM SOLUTION, 1 MMOL IN DMSO* CALCEIN-ACETOXYMETHYL ESTER, DIACETATE Calcein AM (Calcein acetoxymethyl ester) Calcein-AM - CAS 148504-34-1 - Calbiochem Calcein, AM *UltraPure grade* *CAS 148504-34-1* calcein o,o'-diacetate tetrakis(acetoxymethyl)ester Calcein O,o0-diacetate Tetrakis(acetoxymethyl) ester 4',5'-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester Cellstain Calcein AM Solution(1mM/l DMSO) (3',6'-Di(O-acetyl)-4',5'-bis[N,N-bis(carboxymethy 3',6'-DI(O-ACETYL)-2',7'-BIS(N,N-BIS(CARBOXYMETHYL)-AMINOMETHYL)FLUORESCEIN-TETRAACETOXYMETHYL ESTER 3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution Calcein-AM (3',6'-Di(O-acetyl)-2',7'-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceinTetraacetoxymethyl Ester) Glycine, N,N'-((3',6'-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1'-Bis[(acetyloxy) Methyl] Ester Glycine, N,n0-[[30,60-bis(acetyloxy)-3-oxospiro[ isobenzofuran-1(3h),90-[9h]xanthene]-20,70-diyl] bis(methylene)]bis[n-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, bis[(acetyloxy)methyl] Ester Glycine, N,N'-[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-, 1,1'-bis[(acetyloxy)methyl] ester

Chemical Properties

Melting point	>250℃
Boiling point	982.7±65.0 °C(Predicted)
density	1.49±0.1 g/cm3(Predicted)
refractive index	n20/D 1.479
Fp	85 °C
storage temp.	-20°C
solubility	DMSO: soluble10mg/mL, clear, colorless to slightly yellow
form	powder
pka	2.66±0.50(Predicted)
color	Colorless
λmax	< 300 nm
Biological Applications	Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; viability assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes;multi-drug resistance probes;treating atherosclerosis,cancer;ischemic disease

Safety Data

Hazard Codes	Xi
Risk Statements	36/37/38 less more
Safety Statements	26-36 less more
RIDADR	NA 1993 / PGIII
WGK Germany	3
F	10-21

Hazard Information

Uses

A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule

Uses

Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.

Uses

Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.

Definition

ChEBI: Calcein am is an organic heteropentacyclic compound that is calcein in which all four carboxy group hydrogens have been substituted by (acetyloxy)methoxy groups and the hyrodgens of the two hydroxy groups have been substituted by acetyl groups. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases. It has a role as a fluorochrome. It is an acetate ester, an organic heteropentacyclic compound, a gamma-lactone, an oxaspiro compound, a member of 2-benzofurans and a xanthene dye. It is functionally related to a calcein.

General Description

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Biochem/physiol Actions

Fluorescent cell permeable derivative of calcein.

storage

-20°C

Supplier

J & K SCIENTIFIC LTD.

Telephone010-82848833 400-666-7788

Websitehttp://www.jkchemical.com

Beijing Ouhe Technology Co., Ltd

Telephone010-82967028 13552068683

Websitehttp://www.ouhechem.com/

Chemsky（shanghai）International Co.,Ltd.

Telephone021-50135380

Websitehttp://www.shchemsky.com

Chemsky (shanghai) International Co.,Ltd

Telephone021-50135380

Websitehttp://www.shchemsky.com

Dalian Meilun Biotech Co., Ltd.

Telephone0411-62910999 13889544652

Websitehttp://www.meilune.com/

Nanjing Asian Chemical Co., Ltd.

Telephone+86 (25) 82263158

Websitehttps://www.chemicalbook.com/ShowSupplierProductsList13483/0.htm

ShangHai YuanYe Biotechnology Co., Ltd.

Telephone021-61312847 13636370518

Websitehttp://www.shyuanye.com

Shanghai Aladdin Bio-Chem Technology Co.,LTD

Telephone400-6206333 18521732826;

Websitehttp://www.aladdin-e.com/

ShangHai Caerulum Pharma Discovery Co., Ltd.

Telephone 18149758185

Websitehttp://www.caerulumpharma.com

Shanghai Macklin Biochemical Co.,Ltd.

Telephone15221275939 15221275939

Websitehttp://www.macklin.cn

Sigma-Aldrich

Telephone021-61415566 800-8193336

Websitehttps://www.sigmaaldrich.cn

Shanghai EFE Biological Technology Co., Ltd.

Telephone021-65675885 18964387627

Websitehttp://www.efebio.com

Chengdu Dianchun Technology Co., Ltd

Telephone400-1166-196 18502815961

Websitehttp://www.hx-r.com

Beijing Solarbio Science & Tecnology Co., Ltd.

Telephone010-50973130 4009686088

Websitehttp://www.solarbio.com

Beijing Jin Ming Biotechnology Co., Ltd.

Telephone010-60605840 18892239720

Websitehttp://www.jm-bio.com/

Zhengzhou Huiju Chemical Co., Ltd.

Telephone0371-55900031 18137872243

Websitehttps://www.chemicalbook.com/supplier/11124122/

Greenherbs Science and Technology Development Co., Ltd.

Telephone010-61136123

Websitehttp://www.greenherbs.com.cn

Shandong Ono Chemical Co., Ltd.

Telephone0539-6362799 20）

Websitehttp://www.onochem.com

1of4

Related Products

Send Inquriy