143556-24-5

L 012 sodium salt is a luminal-based chemiluminescent dye and probe. It is used to detect superoxide in biological systems, specifically in whole inflammatory cells, vascular tissue and blood. It could detect NAPDH oxidase-derived reactive oxygen and nitrogen species (ROS and RNS). It is active in vitro and in vivo.

L 012 Sodium Salt is a chemiluminescent dye used to detect superoxide in biological systems, specifically in whole blood, inflammatory cells, and vascular tissue.

L-012 is a luminol analog and a widely used reactive oxygen and nitrogen species (RONS; ROS & RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo. L-012 displays significantly higher CL yield and sensitivity than luminollucigenin and MCLA. Instead of reacting with superoxide anion (O2-) directlyL-012 (LumH2) is converted via a one-electron oxidation (catalyzed by peroxidase in the presence of H2O2) to the LumH· radical formwhich then reacts with oxygen (O2) to yield O2- and L-012 quinone (q-Lum). The O2- in turn reacts with the LumH· radicalleading eventually to an endoperoxide th at decomposes to emit luminescence. To a less extendH2O2 can also react with q-Lum to yield luminescence. Although not specific to NADPH oxidase-derived RONSL-012 is also commonly used for screening NADPH oxidase inhibitors.

Store at -20°C