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4-Thiouridine (4-SU) is a photoactivatable ribonucleoside analog that is widely used for RNA analysis, including short-range RNA-RNA crosslinking and nascent RNA labeling. The crosslinking thio moiety is attached directly to the nucleotide base, thus 4-SU differs from uridine only by a single sulfur substitution. This offers the advantage of incorporating into an RNA chain with minimal structural perturbation and with similar base-pairing properties, reducing the likelihood that substitution will impair RNA interactions or activities.

4-Thiouridine is a photoreactive (crosslinking) uridine analogue that upon phosphorylation to 4-thioUTP may be incorporated into RNA structures. 4-Thiouridine may be used to study the specificity and kinetics of uridine-cytidine kinases.

Nucleotide analogue, essential for cell growth in certain bacterial species. This compound is also able to chelate with certain metal ions, and in tRNA it can act as a built-in antiphotomutagenic agent that protects Escherichia coli cells against mutagenesis.

ChEBI: A thiouridine in which the oxygen replaced by sulfur is that at C-4.

4-Thiouridine plays a role in assessing nascent RNA synthesis. It also enhances site-specific crosslinking. 4-Thiouridine also acts as a photoaffinity probe due to its stability even while lacking specific photoactivation. It can also be incorporated into RNAs.

Store at -20°C

[1] KASPAR BURGER. 4-thiouridine inhibits rRNA synthesis and causes a nucleolar stress response.[J]. RNA Biology, 2013, 10 10: 1623-1630. DOI: 10.4161/rna.26214
[2] GILLES THOMAS  Alain F. 4-Thiouridine Triggers Both Growth Delay Induced by Near-Ultraviolet Light and Photoprotection[J]. The FEBS journal, 1980, 113 1: 67-74. DOI: 10.1111/j.1432-1033.1980.tb06140.x
[3] MICHAEL E HARRIS E L C. RNA crosslinking methods.[J]. Methods in enzymology, 2009, 468: 127-146. DOI: 10.1016/s0076-6879(09)68007-1